HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]
Specifications
| Product Data | |
| Clone Name | 4B4 |
|---|---|
| Application | IF, IP, WB |
| Recommended Dilution | Western blot: 1µg/ml was sufficient for ECL immunoblot in 20µg of HeLa lysate. Immunofluorescence. Immunoprecipitation. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rat |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Recombinant mouse HSF1 protein; epitope mapping to amino acids 378-395 |
| Specificity | Highly specific for HSF1. Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots. |
| Buffer | PBS pH 7.4, 50% Glycerol, 0.09% Sodium azide State: Purified State: Liquid |
| Concentration | lot specific |
| Purification | Immunoglobulin fraction purified on Protein G |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | heat shock transcription factor 1 |
| Database Link | |
| Background | HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. |
| Synonyms | HSTF1, HSF-1 |
| Reference Data | |
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