p75 NGF Receptor (NGFR) (1-160) Mouse Monoclonal Antibody [Clone ID: NGFR5]

CAT#: AM00235PU-N

p75 NGF Receptor (NGFR) (1-160) mouse monoclonal antibody, clone NGFR5, Purified

Product Images

Surface staining (mass cytometry) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.

Specifications

Product Data

• Clone Name: NGFR5
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry on Paraffin Sections: 1-2 μg/ml (Heat-mediated antigen retrieval required).
  Positive Control: melanoma tissue.
  Immunocytochemistry.
• Reactivities: Feline, Ferret, Human, Monkey, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified CD271 protein isolated from Human melanoma cell line A875
• Specificity: This antibody recognizes CD271/NGFR, a transmembrane glycoprotein of the TNFR superfamily.
  The epitope is localized within amino acids 1-160.
  Cellular Localization: Cell Surface, Cytoplasmic.
  
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction from Cell Culture Supernatant (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 75 kDa
• Gene Name: nerve growth factor receptor
• Database Link:
  Entrez Gene 4804 Human
  UniProt ID P08138
• Background: CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.
• Synonyms: TNFRSF16