Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]
$495.00
5 Days*
Specifications
| Product Data | |
| Clone Name | PY20 |
|---|---|
| Application | ELISA, IHC, IP, WB |
| Recommended Dilution | Suitable for Immunohistochemistry (5-10 ug/ml) and Immunocytochemistry (5-10 ug/ml). |
| Reactivity | Broad |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein. |
| Specificity | This antibody specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues. |
| Buffer | 0.02 M Sodium Phosphate, pH 7.5 with 0.15 M Sodium Chloride, 3 mM Sodium Azide as preservative, 50% glycerol Label: TAMRA State: Liquid purified IgG fraction. Label: The antibody was labeled by reaction with tetramethyl-rhodamine succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted succinimidyl ester was removed by molecular exclusion chromatography |
| Concentration | lot specific |
| Purification | Affinity purification on a column of immobilized phosphotyrosine. |
| Conjugation | TAMRA |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Background | Phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The rhodamine conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.