Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]
SKU
AM00139PU-N
Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 1E9, Purified
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 1E9 |
|---|---|
| Application | WB |
| Recommended Dilution | Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide conjugated to KLH. |
| Specificity | This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa. |
| Buffer | 1 ml PBS containing 0.09% Sodium Azide as preservative, PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction. |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
| Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | phospholipid scramblase 1 |
| Database Link | |
| Background | Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases. |
| Synonyms | Phospholipid scramblase 1, PL scramblase 1, MmTRA1b |
| Note | Protocol: Positive Control Cell Lysate HepG2 untreated Size Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
| Reference Data | |
| Atlas Protein Categories | Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.