Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]
$540.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 9H8 |
|---|---|
| Application | ELISA, IP, WB |
| Recommended Dilution | ELISA: Use at 0.05 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Included Positive Control: Phosphotyrosine MW standard. |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic phosphopeptide conjugated to KLH Epitope: ...R-G-pY-V-P... |
| Specificity | This antibody recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating positively charged amino acids N-terminal to phosphotyrosine. |
| Buffer | 1 ml 2 x PBS with 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction. |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
| Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Background | Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.