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Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 3B12]

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SKU
AM00125PU-N
Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 3B12, Purified
Size:
100 ug
Conjugation:
Unconjugated Biotin
  $540.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 3B12
Application ELISA, IF, IP, WB
Recommended Dilution Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based
blocking and blot incubation buffer.
ELISA: 0.05 µg/ml
Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells.
Immunocytochemistry: 1 - 10 µg/ml.
Reactivity Canine, Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Specificity Mab PTYR-3B12 recognizes phosphotyrosine in the context of the surrounding amino acids.
Buffer 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
State: Purified
State: Lyophilized purified Ig
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size exclusion chromatography
Conjugation Unconjugated
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Shipping Ambient
Background Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.