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Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: 7A5]

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SKU
AM00099PU-N
Nitrotyrosine mouse monoclonal antibody, clone 7A5, Purified
Size:
100 ug
Conjugation:
Unconjugated
  $540.00
2 Weeks*
Size
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 7A5
Application ELISA, WB
Recommended Dilution Immunoblotting: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 µg/ml.
Reactivity Canine, Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen KLH modified with Nitrotyrosine
Specificity This antibody 7A5 recognizes Nitrotyrosine in proteins.
Buffer 1ml 2 x PBS
State: Purified
State: Lyophilized purified IgG fraction
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore with 1ml H2O (15 min, RT)
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Background The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.
Synonyms NO-Tyrosine, Nitro-Tyrosine
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.