MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G3]

CAT#: AM00091PU-N

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MEK1 (MAP2K1) (incl. pos. control) mouse monoclonal antibody, clone 9G3, Purified



USD 465.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Clone Name 9G3
Applications ELISA, WB
Recommended Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Included Positive ControlCell lysate from untreated HepG2 cells.  (See Protocols)
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml (protein ELISA).
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH
Specificity This antibody specifically recognizes the activation loop of MEK1/2 independent of its phosphorylation status.
Formulation 1 ml PBS / 0.09 % Na-azide / PEG and Sucrose
State: Purified
State: Lyophilized purified Ig
Reconstitution Method Restore with 1 ml H2O (15 min, RT)
Purification Size exclusion chromatography
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Background MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms MAP2K1 - mitogen-activated protein kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1, MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2
Note Protocol: Positive Control: Cell lysate from untreated HepG2 cells
Formulation: Lyophilized cell lysate from serum starved HepG2 cells
Reconstitution: Reconstitute by addition of 200 μl H2O. After complete solubilization add
200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min.
Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Application: The positive control cell lysate is recommended for immunoblot applications.
20 μl of positive control cell lysate correspond to ca. 80.000 cells.
Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for
applications with native proteins such as immunoprecipitation.

Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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