MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E10]

CAT#: AM00090PU-N

MEK1 (MAP2K1) (incl. pos. control) mouse monoclonal antibody, clone 7E10, Purified

Product Images

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MEK1/2-7E10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 7E10
• Applications: ELISA, WB
• Recommended Dilution: Suitable for Western Blot (0.5 μg/ml for HRPO/ECL detection)
  Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Use SW480 pervanadate-treated as positive control (see "Protocols" below).
  ELISA: 0.1 µg/ml.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to KLH.
  Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2
• Specificity: AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.
• Formulation: 1 x PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized
• Reconstitution Method: Reconstitute with 1 ml H2O (15 min, RT)
• Purification: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
• Conjugation: Unconjugated
• Storage: For long-term storage, freeze lyophilizate upon arrival (-20°C).
  Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  Entrez Gene 5604 Human
  UniProt ID Q02750
• Background: MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
• Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1
• Note: A positive control cell lysate is provided with this antibody.
  
  Protocol: Contains Positive Control (pervanadate treated SW 480 cells)
  
  Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for 15min with pervanadate.
  
  Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins.
  
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
  
  Aliquot and store frozen. Avoid repeated freeze/thaw cycles.