malE Mouse Monoclonal Antibody [Clone ID: 6E4]
Specifications
| Product Data | |
| Clone Name | 6E4 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. |
| Reactivity | Escherichia coli |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Purified MBP (Maltose Binding Protein) |
| Specificity | This antibody can be used for the detection of MBP fusion proteins. |
| Buffer | 1ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT) |
| Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid Nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Predicted Protein Size | 43 kDa |
| Database Link | |
| Background | Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins. |
| Synonyms | MMBP, Maltodextrin-binding protein, malE-Tag |
| Reference Data | |
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