Insulin Receptor (INSR) pTyr1322 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 21G12]
SKU
AM00076PU-N
Insulin Receptor (INSR) pTyr1322 (incl. pos. control) mouse monoclonal antibody, clone 21G12, Purified
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 21G12 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from pervanadate-treated HEK-293 cells. ELISA: 0.1 µg/ml. |
| Reactivity | Canine, Human, Mouse |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic phosphopeptide conjugated to KLH. Epitope: Phosphotyrosine 1322 (HIPpYTHM) |
| Specificity | This antibody specifically recognizes Insulin receptor phosphorylated at Tyrosine 1322. |
| Buffer | 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
| Purification | Size Exclusion Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | insulin receptor |
| Database Link | |
| Background | The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes. |
| Synonyms | Insulin Receptor |
| Note | Protocol: Positive Control: Cell lysate from pervanadate-treated HEK-293 cells. Format: Lyophilized cell lysate from HEK-293 cells. Serum starved cells were treated for 15 minutes with pervanadate. Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.