GSK3 beta (GSK3B) pSer9 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D3]

CAT#: AM00068PU-N

GSK3 beta (GSK3B) pSer9 (incl. pos. control) mouse monoclonal antibody, clone 2D3, Purified

Product Images

Phosphospecificity Whole cell extracts of control (co) and pervanadate (VH) treated A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK3b-2D3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 2D3
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Positive Control: Cell lysate from pervanadate treated HepG2 cells.
  ELISA: 0.05 µg/ml.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically recognizes GSK3beta phosphorylated at Ser 9 at 46 kDa. The antibody does not crossreact with GSK3alpha pSer21.
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Size
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  Entrez Gene 2932 Human
  UniProt ID P49841
• Background: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B