GSK3 beta (GSK3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B9]
SKU
AM00066PU-N
GSK3 beta (GSK3B) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 11B9, Purified
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 11B9 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | ELISA: 0.05 µg/ml. Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Postive Control. Cell lysate from untreated HepG2 cells (See Protocols for more details). |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic phosphopeptide conjugated to KLH. |
| Specificity | This antibody specifically recognizes GSK3beta at 46 kDa and does not crossreact with GSK3alpha. |
| Buffer | 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
| Purification | Size Exclusion Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | glycogen synthase kinase 3 beta |
| Database Link | |
| Background | Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling. |
| Synonyms | GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B |
| Note | Protocol: Positive Control: Cell lysate from untreated HepG2 cells Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.