GSK3 alpha (GSK3A) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C1]
SKU
AM00064PU-N
GSK3 alpha (GSK3A) (incl. pos. control) mouse monoclonal antibody, clone 10C1, Purified
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 10C1 |
|---|---|
| Application | WB |
| Recommended Dilution | Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunocytochemistry. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details). |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Peptide conjugated to hemocyanin |
| Specificity | This antibody specifically recognizes GSK3-alpha phosphorylation independent at 51 kDa. Mab GSK3a-10C1 does not crossreact with GSK3-beta. |
| Buffer | PBS containing 0.09% Sodium Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction. |
| Concentration | lot specific |
| Purification | Subsequent Ultrafiltration and Size Exclusion Chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 51 kDa |
| Gene Name | glycogen synthase kinase 3 alpha |
| Database Link | |
| Background | Glycogen Synthase Kinase 3 (GSK3) exists in two isoforms, alpha and beta (GSK3alpha, GSK3beta). GSK3 is a serine/threonine kinase that becomes phosphorylated by PKB/akt upon activation of the PI3K signal transduction pathway. Phosphorylation of GSK3alpha at serine 21 or GSK3beta at serine 9, respectively, leads to inhibition of kinase activity of GSK3. |
| Synonyms | Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A |
| Note | Protocol: Positive Control Provided: Cell lysate from untreated SKOV-3 cells Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
| Reference Data | |
Reviews
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.