N-Acetylglucosamine (GlcNAc) Mouse Monoclonal Antibody [Clone ID: 10D8]

CAT#: AM00063PU-N

N-Acetylglucosamine (GlcNAc) mouse monoclonal antibody, clone 10D8, Purified

Specifications

Product Data

• Clone Name: 10D8
• Applications: ELISA, WB
• Recommended Dilution: Western blot: 0.5 μg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.1 - 1.0 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: N-Acetylglucosamine conjugated to KLH.
• Specificity: This antibody reacts to N-Acetylglucosamine.
• Formulation: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgM
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt store lyophilized (preferably in a desiccator) at -20°C for long-term.
  After reconstitution aliquote and freeze (in liquid nitrogen) at -80°C up to 1 year.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Background: Many intracellular proteins contain monomeric O - linked N - acetylglucosamine residues conjugated to th hydroxyl group of serine or threonine residues. This modification can interfere with tserine and threonine phosphorylation.