FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 6B9 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | ELISA: 0.1 µg/ml. Immunoblotting: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated T47D cells (See Protocol below). |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide conjugated to KLH. |
| Specificity | This antibody specifically recognizes fer at 90 kDa. |
| Buffer | 1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT) |
| Purification | Subsequent Ultrafiltration and Size Exclusion Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch |
| Shipping | Ambient |
| Gene Name | FER tyrosine kinase |
| Database Link | |
| Background | Fer is a non-receptor tyrosine kinase that is widely expressed in different tissues and cell lines. Fer is a homologue of the FES tyrosine kinase that is primarily expressed in cells of hematopoietic origin. |
| Synonyms | c-FER, p94-FER, Tyrosine kinase 3 |
| Note | Protocol: Positive Control: Cell lysate from untreated T47D cells. Format: Lyophilized cell lysate from serum starved T47D cells. Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.