TPTEP2-CSNK1E (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4D7]
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 4D7 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | ELISA: 0.1 µg/ml. Western blot: Use at 0.5 µg/ml for HRPO/ECL detection. Detects a band at 45kD. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from Untreated A431 cells. |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin |
| Specificity | Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1. |
| Buffer | 1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Reconstitute with 1 ml H2O (15 min, RT) |
| Concentration | lot specific |
| Purification | Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | TPTEP2-CSNK1E readthrough |
| Database Link | |
| Background | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling. |
| Synonyms | CKI-epsilon, CKIe, CSNK1E |
| Note | The lyophilized cell lysates (see below) contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. Protocol: Positive Control: Cell lysate from Untreated A431 cells. Format: Lyophilized cell lysate from Serum starved A431 cells Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.