BCL10 pSer138 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6D3]
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 6D3 |
|---|---|
| Application | WB |
| Recommended Dilution | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from serum starved SW480 cells (please see: 'Protocols'). |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Phosphopeptide conjugated to KLH |
| Specificity | This antibody specifically recognizes bcl-10 phosphorylated at Serine 138 at 32 kDa in Western blot. |
| Buffer | 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction. |
| Reconstitution Method | Restore with 1 ml water (15 min, RT). |
| Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | B-cell CLL/lymphoma 10 |
| Database Link | |
| Background | Bcl-10 is an apoptosis-inducing molecule interacting with caspase 9. Bcl-10 enhances pro-caspase 9 processing and induces apoptosis through caspase 9 activation. |
| Synonyms | BCL10, CIPER, CLAP, cCARMEN, mE10, c-E10, hCLAP |
| Note | Molecular Weight: 32 kDa Protocol: Positive Control Cell Lysate: SW 480 Untreated Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
| Reference Data | |
| Atlas Protein Categories | Cytokines, Immune system diseases, Intracellular Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.