Amyloid beta (free N-term, low APP reactive) Mouse Monoclonal Antibody [Clone ID: 19H11]
SKU
AM00007PU-N
Amyloid beta (free N-term, low APP reactive) mouse monoclonal antibody, clone 19H11, Purified
$540.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 19H11 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | Western Blot: 1 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 μg/ml. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | N-terminal peptide conjugated to KLH. |
| Specificity | This antibody recognizes the free N-Terminus of the βA4 polypeptide with high preference and shows only minor crossreactions with APP. |
| Buffer | 1 ml 2 x PBS / 0.1 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT) |
| Purification | Size exclusion chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Background | The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.