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OriGene TissueScan in recent publications
Circulating Adiponectin Is Inversely Associated with Risk of Thyroid Cancer: In Vivo and in Vitro Studies J. Clin. Endocrinol. Metab., Dec 2011; 96: E2023 - E2028. [four normal thyroid tissue specimens ]
QKI-Mediated Alternative Splicing of the Histone Variant MacroH2A1 Regulates Cancer Cell Proliferation Mol. Cell. Biol., Oct 2011; 31: 4244 - 4255. [Breast Tissue RNA]
Performance and clinical evaluation of the 92-gene real-time PCR assay for tumor classification. J Mol Diagn. 2011 Sep;13(5):493-503. [Tumor Tissue]
Inhibition of miR-193a Expression by Max and RXRa Activates K-Ras and PLAU to Mediate Distinct Aspects of Cellular Transformation Cancer Res., Aug 2011; 71: 5144 - 5153. [RNAs from colon adenocarcinomas  ]

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ProteoScan Cancer Lysate Array Specifications

Reverse-Phase Protein Arrays

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FeatureCancer Lysate Arrays – Cat. # PA100002 Assay Validation Arrays – Cat. # PA100001
Summary
  • 432 unique human tissues, covering 11 different cancer types
  • For each cancer type, there are 25 different cancer tissues and 15 normal tissues
  • Each sample is spotted in triplicate in 4 serial dilutions
  • 44 unique human tissues, covering 11 different tissue types
  • For each cancer, there are 2 different cancer tissues and 2 normal tissues
  • Each sample is spotted in triplicate at 1000 ug/ml
  • Ideal for antibody/reagent validation and optimization
General array layout schemepdf pdf
Download Sample ListComplete tissue lysate sample list and orientation
Complete tissue sample clinical data		 Sample list and orientation
Slide TypeGlass slide coated with a thin layer of nitrocellulose, 1 x 3” (25mm x 75mm) Glass slide coated with a thin layer of nitrocellulose, 1 x 3” (25mm x 75mm)
Nitrocellulose length60 mm51 mm
Total Features/Array6,603201
Number of Cancer Tissue Spots3240 (270 X 4 X3)66 (22 X3)
Number of Normal Tissue Spots1944 (162 X 4 X3)66 (22 X3)
Tissue Lysate Protein Concentration (ug/ml)1000, 500, 200, and 1001000
Total Amount Protein per Spot (ng)About 0.03 - 0.3~ 0.3
Positive Controls924 (IgG mix 231, b-actin 132, cell lysate over expressed + control 264; BSA-C3, BSA-C5, BSA-C3-C5) 54 (IgG mix 21, cell lysate over expressed + control 24; BSA-C3, BSA-C5, BSA-C3-C5)
Negative Controls (ug/ml)495 (BSA 1000, 500, 200, 100, buffer) 15 (BSA 1000, 500, 200, 100, buffer)
PrintingDirect contact pin spotting Pin size 110 uM Direct contact pin spotting Pin size 110 uM
Number of subarray331
Subarray Size4.5 μm × 4.5 μm22.5 μm × 14.2 μm
Total Array Size40.5 μm × 18 μm22.5 μm × 14.2 μm
Median Spot Diameter200 μm200 μm
Spot Center to Center spacing300 μmX-axis 1,000 μm
Y-axis 1,500 μm
Kit Composition• ProteoScan Cancer Lysate Array 1.0 (2 slides)
• Instruction Manual		• ProteoScan Assay Validation Array (2 slides)
• Instruction Manual
Permitted UseResearch Use OnlyResearch Use Only

 

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