Q: What are TissueScan qPCR Arrays?
A: TissueScan is a gene-expression profiling tool. It consists of a panel of normalized first strand cDNA from multiple (12-48) tissues serving as template for gene-specific PCR amplification and therefore reveals the gene expression profile among different organ/tissues/developmental stages.
Q: What are the advantages of TissueScan qPCR Array compared to traditional Northern Blot?
A: There are several advantages of using TissueScan qPCR Array compared to the traditional Northern blot method.
Wider spectrum of tissue/organ: The TissueScan qPCR Array consists of cDNA from 12-48 different tissues/organs. Traditional Northern consists of 4-12 lanes.
Easy and quick procedure: TissueScan provides normalized cDNA arrayed in 96-well PCR-ready plate. The complete procedure includes a single qPCR reaction with gene-specific primers. The complete procedure takes less than 2 hrs with less than 10 minutes hands-on time.
Increased sensitivity: TissueScan is a qPCR based method that allows detection of rare transcripts that may be beneath the detection limit of the Northern blot technique.
Enhanced specificity: TissueScan detects transcripts based upon specificity of primers which is better than hybridization based Northern Blot methods.
Q: Are the cDNA from a single donor or a pool of donors?
A: The cDNAs are derived from a pool of at least 5 donors.
Q: Which species of mouse and rat are used to make the TissueScan Arrays?
A: The mouse species is Swiss Webster and the rat species is Sprague Dawley.
Q: Which housekeeping genes are used for normalization?
A: For all mouse, human and rat TissueScan, GAPDH is used for the normalization. For the drosophila panel, rp49 is used.
Q: Which thermocyclers are compatible with TissueScan qPCR Arrays?
A: TissueScan are made with PCR plates from Abgene (AB-0600). According to the plate manufacturer, the plates will fit in the following machines: Standard Thermocyclers
Biometro Uno, Uno II, T1, Tgradient, TRobot
BioRad iCycler and MyCycler
Eppendorf Mastercycler Gradient and Mastercycler EP Gradient
Ericomp SingleBlock system, TwinBlock system, and Deltacycler I
ThermoHybaid PCR Express, Px2, PxE, MultiBlock System and MBS, Touchdown, Omnigene, and Omn-E
MJ Research PTC-200 DNA Engine, PTC-225 DNA Tetrad/PTC-220/221 DNA Dyad, and PTC-100 with 96 well block
MWG Primus 96 and TheQ Lifecycler
ABI GeneAmp 2700/2720, GeneAmp 9600, and GeneAmp 9700
Techne TC-412/512, Touchdown Gradient, Flexigene, and Genius
ABI Prism 7000, 7700, 7300, 7500, and 9500 (except fast block formats)
BioRad iCycler and MyiQ
Stratagene MX4000, MX3000p, and MX3005p
If your thermocycler is not listed, please contact Technical Support for more information. Another solution (not recommended) is to resuspend the cDNA in the provided plate and transfer it to a plate compatible with your machine. To do so, add half of the reaction volume to each well of the product plate and incubate on ice for 20 minutes. Then transfer the resuspended solution to a plate using a multi-channel pipette, add the remainder of the reaction volume and other reagents, and run your experiment. Exceptionally careful resuspension and pipetting would be mandatory to preserve the quantitative nature of this product, which is why this option is not recommended.
Q: What is included in one TissueScan panel?
A: Other than the gene-specific primers, the TissueScan Gene Expression Panel kit provides all the essential items for convenient application.
Two identical, sealed, 96-well format plates containing dried, serially-diluted, PCR-ready, first-strand cDNAs
Two adhesive cover sheets for sealing the plates prior to performing PCR
Control primer pair of housekeeping gene for normalization
Q: What kinds of panels are available?
A: Currently OriGene provides the following panels:
Human Major TissueScan™ Panel (48 Tissues) HMRT102
Human Brain TissueScan™ Panel (24 Parts) HBRT101
Mouse Normal TissueScan ™ Panel (36 Tissues) MNRT101
Drosophila Normal and Embryo TissueScan™ Panel (12 Parts/Stages) DSRT101
Rat Normal TissueScan™ Panel (46 Tissues) RNRT101
Q: I ordered the Tissue and the 96-well plate looked dry. Did you send me the empty plates by mistake?
A: No. The dried plates provided contain lyophilized cDNA on the bottom of each well.
Q: I need to cite your product for a paper I am writing. What language should I use?
A: We recommend that you refer to the product by its specific catalog number and refer to us as OriGene Technologies (Rockville, MD). Furthermore, we'd love to hear from you when your paper is published. Inform us and we will send a gift.