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TrueClone cDNA Clones
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OriGene cDNA clone collection in recent publications
An Unbiased Approach to Identifying Tau Kinases That Phosphorylate Tau at Sites Associated with Alzheimer Disease J. Biol. Chem., Aug 2013; 288: 23331 - 23347. [GFC Kinase Array]

Transmembrane Protein 214 (TMEM214) Mediates Endoplasmic Reticulum Stress-induced Caspase 4 Enzyme Activation and Apoptosis J. Biol. Chem., Jun 2013; 288: 17908 - 17917. [10,000 Clone Collection]

Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers Cell. 2013 May 23;153(5):963-75 [10,000 Clone Collection]

RNF34 Is a Cold-Regulated E3 Ubiquitin Ligase for PGC-1a and Modulates Brown Fat Cell Metabolism Mol. Cell. Biol., Jan 2012; 32: 266 - 275. [264 cDNA expression clones]

Normal Tissue TissueScan FAQs

What are TissueScan Normal qPCR Array?
What are the advantages of TissueScan comparing to traditional Northern Blot?
Are the cDNA from a single donor or a pool of donors?
Which species of mouse and rat are used to make the TissueScan Arrays?
Which housekeeping genes are used for normalization
Which thermocyclers are compatible with TissueScan qPCR Arrays?
What is included in one TissueScan panel?
What kinds of panels are available?
I ordered the TissueScan and the 96-well plate looked dry. Did you send me the empty plates by mistake? I need to cite your product for a paper I am writing. What language should I use?

Q: What are TissueScan qPCR Arrays?
A:
TissueScan is a gene-expression profiling tool. It consists of a panel of normalized first strand cDNA from multiple (12-48) tissues serving as template for gene-specific PCR amplification and therefore reveals the gene expression profile among different organ/tissues/developmental stages.

Q: What are the advantages of TissueScan qPCR Array compared to traditional Northern Blot?
A:
There are several advantages of using TissueScan qPCR Array compared to the traditional Northern blot method.

  • Wider spectrum of tissue/organ: The TissueScan qPCR Array consists of cDNA from 12-48 different tissues/organs. Traditional Northern consists of 4-12 lanes.
  • Easy and quick procedure: TissueScan provides normalized cDNA arrayed in 96-well PCR-ready plate. The complete procedure includes a single qPCR reaction with gene-specific primers. The complete procedure takes less than 2 hrs with less than 10 minutes hands-on time.
  • No radioactivity.
  • Increased sensitivity: TissueScan is a qPCR based method that allows detection of rare transcripts that may be beneath the detection limit of the Northern blot technique.
  • Enhanced specificity: TissueScan detects transcripts based upon specificity of primers which is better than hybridization based Northern Blot methods.

Q: Are the cDNA from a single donor or a pool of donors?
A:
The cDNAs are derived from a pool of at least 5 donors.

Q: Which species of mouse and rat are used to make the TissueScan Arrays?
A:
The mouse species is Swiss Webster and the rat species is Sprague Dawley.

Q: Which housekeeping genes are used for normalization?
A:
For all mouse, human and rat TissueScan, GAPDH is used for the normalization. For the drosophila panel, rp49 is used.

Q: Which thermocyclers are compatible with TissueScan qPCR Arrays?
A:
TissueScan are made with PCR plates from Abgene (AB-0600). According to the plate manufacturer, the plates will fit in the following machines:
Standard Thermocyclers

  • Biometro Uno, Uno II, T1, Tgradient, TRobot
  • BioRad iCycler and MyCycler
  • Eppendorf Mastercycler Gradient and Mastercycler EP Gradient
  • Ericomp SingleBlock system, TwinBlock system, and Deltacycler I
  • ThermoHybaid PCR Express, Px2, PxE, MultiBlock System and MBS, Touchdown, Omnigene, and Omn-E
  • MJ Research PTC-200 DNA Engine, PTC-225 DNA Tetrad/PTC-220/221 DNA Dyad, and PTC-100 with 96 well block
  • MWG Primus 96 and TheQ Lifecycler
  • ABI GeneAmp 2700/2720, GeneAmp 9600, and GeneAmp 9700
  • Stratagene Robocycler
  • TaKaRa TP3000
  • Techne TC-412/512, Touchdown Gradient, Flexigene, and Genius
qPCR Thermocyclers
  • ABI Prism 7000, 7700, 7300, 7500, and 9500 (except fast block formats)
  • BioRad iCycler and MyiQ
  • Stratagene MX4000, MX3000p, and MX3005p
If your thermocycler is not listed, please contact Technical Support for more information. Another solution (not recommended) is to resuspend the cDNA in the provided plate and transfer it to a plate compatible with your machine. To do so, add half of the reaction volume to each well of the product plate and incubate on ice for 20 minutes. Then transfer the resuspended solution to a plate using a multi-channel pipette, add the remainder of the reaction volume and other reagents, and run your experiment. Exceptionally careful resuspension and pipetting would be mandatory to preserve the quantitative nature of this product, which is why this option is not recommended.

Q: What is included in one TissueScan panel?
A:
Other than the gene-specific primers, the TissueScan Gene Expression Panel kit provides all the essential items for convenient application.

  • Two identical, sealed, 96-well format plates containing dried, serially-diluted, PCR-ready, first-strand cDNAs
  • Two adhesive cover sheets for sealing the plates prior to performing PCR
  • Control primer pair of housekeeping gene for normalization

Q: What kinds of panels are available?
A:
Currently OriGene provides the following panels:

  • Human Major TissueScan™ Panel (48 Tissues) HMRT102
  • Human Brain TissueScan™ Panel (24 Parts) HBRT101
  • Mouse Normal TissueScan ™ Panel (36 Tissues) MNRT101
  • Mouse Developmental TissueScan™ Panel (48 Parts/Stages) MDRT101
  • Drosophila Normal and Embryo TissueScan™ Panel (12 Parts/Stages) DSRT101
  • Rat Normal TissueScan™ Panel (46 Tissues) RNRT101

Q: I ordered the Tissue and the 96-well plate looked dry. Did you send me the empty plates by mistake?
A:
No. The dried plates provided contain lyophilized cDNA on the bottom of each well.

Q: I need to cite your product for a paper I am writing. What language should I use?
A:
We recommend that you refer to the product by its specific catalog number and refer to us as OriGene Technologies (Rockville, MD). Furthermore, we'd love to hear from you when your paper is published. Inform us and we will send a gift.

 

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