ExactORF Service FAQs

Am I assured that the clone I receive will match the nucleotide sequence I entered?
Answer: Absolutely.  We will use every technology at OriGene to make sure that the exact nucleotide sequence is created and verified.

What quality of sequence do you provide on these clones?
Answer: OriGene uses quality ABI reagents and the newest ABI3730 DNA sequencer.  Both nucleotide strands across the insert and cloning junctions are covered by reads whose quality is checked using the PHRED algorithm.

What will I receive in terms of sequence confirmation?
Answer: All chromatograms, a consensus sequence file and a vector map will be included on a USB-Flash drive for easy upload to your computer.

How much DNA will I receive?
Answer: 10ug; lyophilized into the bottom of a Matrix-brand 2D bar-coded screw-cap tube.  This should be plenty for most applications without the need to re-transform, select a colony and re-purify.  You can also ask OriGene to provide you with more DNA at the time of order.

How much more does OriGene charge per bp for larger sizes or GC-richness?
Answer:  There are no hidden surcharges with the ExactORF service.  Many other providers need to charge for this to offset the costs they have using their technology.  This is not the case at OriGene.

Why does it take so long to deliver?
Answer:  As a custom service, specialized care in project design and quality assurance takes time.  All clones are different and it is quite possible that you receive your clone ahead of what was predicted.  However, we wish to over-deliver, and not over-sell.  Please contact custsupport@origene.com for updates.

What vector will be used to clone my ORF?
Answer:  You may select from any vector that OriGene offers from our PrecisionShuttle vector system.  OriGene offers vectors to over-express your protein or domain in the native form or tagged with a host of fusion partners.

What will I receive in the box?
Answer: 10ug of the final plasmid DNA (ion-exchange grade for immediate transfection); all chromatograms in the .ab1 file format; a vector map; a certificate of analysis and Application Guide.

Can I dictate which restriction sites to use?
Answer: To maintain reading frames, OriGene PrecisionShuttle vectors are specially designed to use rare cutters.  Your additional sites can be added but may result in additional coding amino acids or may introduce a frameshift.  A trained consultant (contact OriGene technical support) will be happy to discuss your options.

Does OriGene guarantee the protein expression level from this construct?
Answer:  No.  There are too many variables with respect to transfection efficiency, host cells and growth conditions.  However, our techsupport team will help offer suggestions.  OriGene guarantees the correct sequence in the correct vector and cloned appropriately into the specified restriction sites.

Why is OriGene a preferred provider for synthetic clones?
Answer:  OriGene remains “Your Gene Company” and as such it recognizes that the researcher’s need for synthetic constructs is complementary to its world-leading business in supplying naturally occurring TrueClone cDNA transcripts.  Its decade long immersion in the genetics field and its laboratories fully stocked with seasoned PhD-level molecular biologists, together make OriGene the right choice for experience and know-how.