Regeneron Pharmaceuticals Licenses OriGene TrueClone Collection
“We believe that full-length cDNAs isolated from cDNA libraries are the most reliable source of authentic coding regions for our critical applications." said Drew Murphy, Vice President of Target Discovery for Regeneron. "The OriGene True Clone collection has proven to be an excellent source of full-length human cDNAs for use in Regeneron's protein expression and target validation platforms."
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HuSH shRNA Citations
References for HuSH shRNA
- PCPH/ENTPD5 Expression Enhances the Invasiveness of Human Prostate Cancer Cells by a Protein Kinase CD–Dependent Mechanism
Joaquin Villar, Maria Isabel Arenas, Caitlin M. MacCarthy, Maria Jose Blanquez, Oscar M. Tirado, and Vicente Notario Cancer Research, Nov 2007; 67: 10859-10868
- A comprehensive ChIP–chip analysis of E2F1, E2F4, and E2F6 in normal and tumor cells reveals interchangeable roles of E2F family members
Xiaoqin Xu, Mark Bieda, Victor X. Jin, Alina Rabinovich, Mathew J. Oberley, Roland Green, and Peggy J. Farnham Genome Res., Nov 2007; 17: 1550 - 1561
- NHERF1 regulates parathyroid hormone receptor membrane retention without affecting recycling
Bin Wang, Alessandro Bisello, Yanmei Yang, Guillermo G. Romero, and Peter A. Friedman J. Biol. Chem., Sep 2007; 10.1074/jbc.M707263200.
- Alternative splicing yields protein-arginine methyltransferase 1 isoforms with distinct activity, substrate specificity and subcellular localization
Isabelle Goulet, Gabrielle Gauvin, Sophie Boisvenue, and Jocelyn Cote J. Biol. Chem., Nov 2007, 282: 33009-33021
- Tesk1 interacts with sprouty2 to abrogate its inhibition of ERK phosphorylation downstream of receptor tyrosine kinase signaling
Sumana Chandramouli, Chye Yun Yu, Permeen Yusoff, Dieu-Hung Lao, Hwei Fen Leong, Kensaku Mizuno, and Graeme R. Guy J. Biol. Chem., Nov 2007, 10.1074/jbc.M705457200
- Tumor Suppressor Activity of CCAAT/Enhancer Binding Protein Alpha Is Epigenetically Down-regulated in Head and Neck Squamous Cell Carcinoma
Kristi L. Bennett, Björn Hackanson, Laura T. Smith, Carl D. Morrison, James C. Lang, David E. Schuller, Frank Weber, Charis Eng and Christoph Plass Cancer Res., May 2007; 67: 4657
- L-arginine availability regulates T-lymphocyte cell-cycle progression
Paulo C. Rodriguez, David G. Quiceno, and Augusto C. Ochoa Blood, Feb 2007; 109: 1568 - 1573.
- Naked2 Acts as a Cargo Recognition and Targeting Protein to Ensure Proper Delivery and Fusion of TGF-a containing Exocytic Vesicles at the Lower Lateral Membrane of Polarized MDCK Cells
Cunxi Li, Mingming Hao, Zheng Cao, Wei Ding, Ramona Graves-Deal, Jianyong Hu, David W. Piston, and Robert J. Coffey Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E07-02-0172.
- Identification of ERRa as a specific partner of PGC-1a for the activation of PDK4 gene expression in muscle
Makoto Araki and Kiyoto Motojima FEBS Journal, April 2006, 273: 1669-1680
- Src-Family Kinases Are Activated in Non-Small Cell Lung Cancer and Promote the Survival of Epidermal Growth Factor Receptor-Dependent Cell Lines
Jie Zhang, Shailaja Kalyankrishna, Marie Wislez, Nishan Thilaganathan, Babita Saigal, Wei Wei, Long Ma, Ignacio I. Wistuba, Faye M. Johnson, and Jonathan M. Kurie Am J. Pathol., 2007, 170: 366-376
- A Genome-Wide Screen Reveals Functional Gene Clusters in the Cancer Genome and Identifies EphA2 as a Mitogen in Glioblastoma
Fenghua Liu, Peter J. Park, Weil Lai, Elizabeth Maher, Arnab Chakravarti,Laura Durso, Xiuli Jiang, Yi Yu,Amanda Brosius, Meredith Thomas,Lynda Chin, Cameron Brennan, Ronald A. DePinho, Isaac Kohane,Rona S. Carroll,Peter M. Black,and Mark D. Johnson Cancer Research, 2006, 66: 10815-10823
References for shRNA
Mammalian RNAi: a practical guide. P. Sandy, A. Ventura, T. Jacks, Biotechniques. 2005 August, 39, 215-224
Synthetic dsRNA Dicer substrate enhance RNA potency and efficiency. Dong-Ho Kim, Mark A Behlke, Scott D Rose, Mi-Sook Chang, Sangdun Choi & John J Rossi. Nat. Biotechnol. 2004 Dec 26. 23, 222-226
Synthetic shRNAs as potent RNAi triggers. Despina Siolas, Cara Lerner, Julja Burchard, Wei Ge, Peter S Linsley, Patrick J Paddison, Gregory J Hannon & Michele A Cleary. Nat.Biotechnol. 2004 Dec 26. 23, 227-231
Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. Richard J Fish and Egbert KO Kruithof BMC Molecular Biology 2004, 5:9
RNAi-Mediated Silencing of Insulin Receptor Substrate1 (IRS-1) Enhances Tamoxifen-Induced Cell Death inMCF-7 Breast Cancer Cells Gregory Cesarone, Cecilia Garofalo, Marc T. Abrams, Olga Igoucheva, Vitali Alexeev, Kyonggeun Yoon, Eva Surmacz, Eric Wickstrom J Cell Biochem. 2006 May 15;98(2):440-50
Small non-coding RNAs as magic bullets Fritz Eckstein. Trends in Biochemical Sciences, 2005 Aug, 30:8, 445-452.
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