Home siRNA SR308340
CLCF1 (ID 23529) Trilencer-27 Human siRNA
- CLCF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 23529)
- Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
- Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
||SiTran1.0: Transfection reagent designed for RNAi duplex (0.5 ml)
Free! With the purchase of 2 siRNA kits (save $220)
* These siRNA duplexes were designed to be effective against all transcriptional variants at this gene locus.
Also for CLCF1 (Locus ID 23529)
|Amount:||2 nmol each, lyophilized||Purity:||HPLC purified|
|Quality Control:||Tested by ESI-MS||Sequences:||Available with shipment|
|Stability:||One year from date of shipment when stored at -20ºC||Shipment: Ambient|
|# of transfections:||Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)|
|Synonyms: BSF-3; BSF3; CISS2; CLC; NNT-1; NNT1; NR6|
|Summary: This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. |Performance Guranteed:
OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping