OriGene - CLCF1 (NM_013246) siRNA

OriGene Data

Amount:	2 nmol each, lyophilized	Purity:	HPLC purified
Quality Control:	Tested by ESI-MS	Sequences:	Available with shipment
Stability:	One year from date of shipment when stored at -20ºC		Shipment: Ambient
# of transfections:	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)

Reference Data

RefSeq: NM_001166212NM_013246

Synonyms: BSF-3; BSF3; CISS2; CLC; NNT-1; NNT1; NR6

Summary: This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009].

Performance Guranteed:

OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

siRNA Citations:

IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation, Lue Dai, Kristy B. Lidie, Qian Chen, Joseph W. Adelsberger, Xin Zheng, DaWei Huang, Jun Yang, Richard A. Lempicki, Tauseef Rehman, Robin L. Dewar, Yanmei Wang, Ronald L. Hornung, Kelsey A. Canizales, Stephen J. Lockett, H. Clifford Lane, and Tomozumi Imamichi, J. Exp. Med., Mar 2013; 210: 517 - 534. [SPTBN1 ]

Protein Kinases D2 and D3 Are Novel Growth Regulators in HCC1806 Triple-negative Breast Cancer Cells, QIN HAO, RAYMOND MCKENZIE, HUACHEN GAN, and HUA TANG, Anticancer Res, Feb 2013; 33: 393 - 399. [PRKCA]

Targeting the Metastasis Suppressor, NDRG1, Using Novel Iron Chelators: Regulation of Stress Fiber-Mediated Tumor Cell Migration via Modulation of the ROCK1/pMLC2 Signaling Pathway, Jing Sun, Daohai Zhang, Ying Zheng, Qian Zhao, Minhua Zheng, Zaklina Kovacevic, and Des R. Richardson, Mol. Pharmacol., Feb 2013; 83: 454 - 469. [ROCK1 ]

Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation , Zhang ZR, Al Zaharna M, Wong MM, Chiu SK, Cheung HY., PLoS One. 2013;8(1):e54577 [MAD2L1 ]

miR-23b Represses Proto-oncogene Src Kinase and Functions as Methylation-Silenced Tumor Suppressor with Diagnostic and Prognostic Significance in Prostate Cancer, Shahana Majid, Altaf A. Dar, Sharanjot Saini, Sumit Arora, Varahram Shahryari, Mohd Saif Zaman, Inik Chang, Soichiro Yamamura, Yuichiro Tanaka, Guoren Deng, and Rajvir Dahiya, Cancer Res., Dec 2012; 72: 6435 - 6446. [SRC ]

cAMP-responsive Element Modulator a (CREMa) Contributes to Decreased Notch-1 Expression in T Cells from Patients with Active Systemic Lupus Erythematosus (SLE), Thomas Rauen, Alexandros P. Grammatikos, Christian M. Hedrich, Jürgen Floege, Klaus Tenbrock, Kim Ohl, Vasileios C. Kyttaris, and George C. Tsokos, J. Biol. Chem., Dec 2012; 287: 42525 - 42532. [NOTCH1]

The p53 target gene desmocollin 3 acts as a novel tumor suppressor through inhibiting EGFR/ERK pathway in human lung cancer, Tiantian Cui, Yuan Chen, Linlin Yang, Thomas Knösel, Otmar Huber, Manuela Pacyna-Gengelbach, and Iver Petersen, Carcinogenesis, Dec 2012; 33: 2326 - 2333. [DSC3]

A possible gene silencing mechanism: Hypermethylation of the Keap1 promoter abrogates binding of the transcription factor Sp1 in lung cancer cells , D Guo, B Wu, J Yan, X Li, H Sun, D Zhou, Biochem Biophys Res Commun. 2012 Nov 9;428(1):80-5 [SP1]

cAMP response element modulator a controls IL2 and IL17A expression during CD4 lineage commitment and subset distribution in lupus, Christian M. Hedrich, Jose C. Crispin, Thomas Rauen, Christina Ioannidis, Sokratis A. Apostolidis, Mindy S. Lo, Vasileios C. Kyttaris, and George C. Tsokos, PNAS, Oct 2012; 109: 16606 - 16611. [DNMT3a]

Desmoplakin acts as a tumor suppressor by inhibition of the Wnt/ß-catenin signaling pathway in human lung cancer, Linlin Yang, Yuan Chen, Tiantian Cui, Thomas Knösel, Qing Zhang, Kai Frederik Albring, Otmar Huber, and Iver Petersen, Carcinogenesis, Oct 2012; 33: 1863 - 1870. [DSP]

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