Home siRNA SR304341
SFTPB (ID 6439) Trilencer-27 Human siRNA
- SFTPB (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 6439)
- Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
- Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
- SiTran1.0: Transfection reagent designed for RNAi duplex (0.5 ml)
* These siRNA duplexes were designed to be effective against all transcriptional variants at this gene locus.
** Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping
Also for SFTPB (Locus ID 6439)
|Amount: 2 nmol each, lyophilized||Purity: HPLC purified|
|Quality Control: Tested by ESI-MS||Sequences: Available with shipment|
|Stability: One year from date of shipment when stored at -20ºC||Shipment: Ambient|
|# of transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)|
|Note: Single siRNA duplex (10nmol) can be ordered. See details at http://www.origene.com/siRNA/|
|Synonyms: PSP-B; SFTB3; SFTP3; SMDP1; SP-B|
|Summary: This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]. |Performance Guranteed:
OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).