OriGene - MPL (NM_005373) siRNA

OriGene Data

Amount: 2 nmol each, lyophilized	Purity: HPLC purified
Quality Control: Tested by ESI-MS	Sequences: Available with shipment
Stability: One year from date of shipment when stored at -20ºC	Shipment: Ambient
# of transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)
Note: Single siRNA duplex (10nmol) can be ordered. See details at http://www.origene.com/siRNA/

Reference Data

RefSeq: NM_005373

Synonyms: C-MPL; CD110; MPLV; THCYT2; TPOR

Summary: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul

Performance Guranteed:

OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

siRNA Citations:

Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy, Yonghua Yang, Warren Fiskus, Bao Yong, Peter Atadja, Yoshinori Takahashi, Tej K. Pandita, Hong-Gang Wang, and Kapil N. Bhalla, PNAS, Apr 2013; 110: 6841 - 6846. [KAP1 ]

GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells, Jessica L. Schwartz-Roberts, Ayesha N. Shajahan, Katherine L. Cook, Anni Wärri, Mones Abu-Asab, and Robert Clarke, Mol. Cancer Ther., Apr 2013; 12: 448 - 459. [BECN1 ]

SCO2 Induces p53-Mediated Apoptosis by Thr845 Phosphorylation of ASK-1 and Dissociation of the ASK-1–Trx Complex, Esha Madan, Rajan Gogna, Periannan Kuppusamy, Madan Bhatt, Abbas Ali Mahdi, and Uttam Pati, Mol. Cell. Biol., Apr 2013; 33: 1285 - 1302. [p53]

IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation, Lue Dai, Kristy B. Lidie, Qian Chen, Joseph W. Adelsberger, Xin Zheng, DaWei Huang, Jun Yang, Richard A. Lempicki, Tauseef Rehman, Robin L. Dewar, Yanmei Wang, Ronald L. Hornung, Kelsey A. Canizales, Stephen J. Lockett, H. Clifford Lane, and Tomozumi Imamichi, J. Exp. Med., Mar 2013; 210: 517 - 534. [SPTBN1 ]

Protein Kinases D2 and D3 Are Novel Growth Regulators in HCC1806 Triple-negative Breast Cancer Cells, QIN HAO, RAYMOND MCKENZIE, HUACHEN GAN, and HUA TANG, Anticancer Res, Feb 2013; 33: 393 - 399. [PRKCA]

Targeting the Metastasis Suppressor, NDRG1, Using Novel Iron Chelators: Regulation of Stress Fiber-Mediated Tumor Cell Migration via Modulation of the ROCK1/pMLC2 Signaling Pathway, Jing Sun, Daohai Zhang, Ying Zheng, Qian Zhao, Minhua Zheng, Zaklina Kovacevic, and Des R. Richardson, Mol. Pharmacol., Feb 2013; 83: 454 - 469. [ROCK1 ]

Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation , Zhang ZR, Al Zaharna M, Wong MM, Chiu SK, Cheung HY., PLoS One. 2013;8(1):e54577 [MAD2L1 ]

miR-23b Represses Proto-oncogene Src Kinase and Functions as Methylation-Silenced Tumor Suppressor with Diagnostic and Prognostic Significance in Prostate Cancer, Shahana Majid, Altaf A. Dar, Sharanjot Saini, Sumit Arora, Varahram Shahryari, Mohd Saif Zaman, Inik Chang, Soichiro Yamamura, Yuichiro Tanaka, Guoren Deng, and Rajvir Dahiya, Cancer Res., Dec 2012; 72: 6435 - 6446. [SRC ]

cAMP-responsive Element Modulator a (CREMa) Contributes to Decreased Notch-1 Expression in T Cells from Patients with Active Systemic Lupus Erythematosus (SLE), Thomas Rauen, Alexandros P. Grammatikos, Christian M. Hedrich, Jürgen Floege, Klaus Tenbrock, Kim Ohl, Vasileios C. Kyttaris, and George C. Tsokos, J. Biol. Chem., Dec 2012; 287: 42525 - 42532. [NOTCH1]

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MPL (ID 4352) Trilencer-27 Human siRNA

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