OriGene - IL12B (NM_002187) siRNA

OriGene Data

Amount: 2 nmol each, lyophilized	Purity: HPLC purified
Quality Control: Tested by ESI-MS	Sequences: Available with shipment
Stability: One year from date of shipment when stored at -20ºC	Shipment: Ambient
# of transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)
Note: Single siRNA duplex (10nmol) can be ordered. See details at http://www.origene.com/siRNA/

Reference Data

RefSeq: NM_002187

Synonyms: CLMF; CLMF2; IL-12B; NKSF; NKSF2

Summary: This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008].

Performance Guranteed:

OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

siRNA Citations:

CD163 and IgG Codefend against Cytotoxic Hemoglobin via Autocrine and Paracrine Mechanisms, Karthik Subramanian, Ruijuan Du, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding, J. Immunol., May 2013; 190: 5267 - 5278. [FCGR2A ]

The Syndecan-4/Protein Kinase Ca Pathway Mediates Prostaglandin E2-induced Extracellular Regulated Kinase (ERK) Activation in Endothelial Cells and Angiogenesis in Vivo, Federico Corti, Federica Finetti, Marina Ziche, and Michael Simons, J. Biol. Chem., May 2013; 288: 12712 - 12721. [PRKCA ]

Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy, Yonghua Yang, Warren Fiskus, Bao Yong, Peter Atadja, Yoshinori Takahashi, Tej K. Pandita, Hong-Gang Wang, and Kapil N. Bhalla, PNAS, Apr 2013; 110: 6841 - 6846. [KAP1 ]

GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells, Jessica L. Schwartz-Roberts, Ayesha N. Shajahan, Katherine L. Cook, Anni Wärri, Mones Abu-Asab, and Robert Clarke, Mol. Cancer Ther., Apr 2013; 12: 448 - 459. [BECN1 ]

SCO2 Induces p53-Mediated Apoptosis by Thr845 Phosphorylation of ASK-1 and Dissociation of the ASK-1–Trx Complex, Esha Madan, Rajan Gogna, Periannan Kuppusamy, Madan Bhatt, Abbas Ali Mahdi, and Uttam Pati, Mol. Cell. Biol., Apr 2013; 33: 1285 - 1302. [p53]

IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation, Lue Dai, Kristy B. Lidie, Qian Chen, Joseph W. Adelsberger, Xin Zheng, DaWei Huang, Jun Yang, Richard A. Lempicki, Tauseef Rehman, Robin L. Dewar, Yanmei Wang, Ronald L. Hornung, Kelsey A. Canizales, Stephen J. Lockett, H. Clifford Lane, and Tomozumi Imamichi, J. Exp. Med., Mar 2013; 210: 517 - 534. [SPTBN1 ]

Protein Kinases D2 and D3 Are Novel Growth Regulators in HCC1806 Triple-negative Breast Cancer Cells, QIN HAO, RAYMOND MCKENZIE, HUACHEN GAN, and HUA TANG, Anticancer Res, Feb 2013; 33: 393 - 399. [PRKCA]

Targeting the Metastasis Suppressor, NDRG1, Using Novel Iron Chelators: Regulation of Stress Fiber-Mediated Tumor Cell Migration via Modulation of the ROCK1/pMLC2 Signaling Pathway, Jing Sun, Daohai Zhang, Ying Zheng, Qian Zhao, Minhua Zheng, Zaklina Kovacevic, and Des R. Richardson, Mol. Pharmacol., Feb 2013; 83: 454 - 469. [ROCK1 ]

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