Summary: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Mechanosensitive ATP release from hemichannels and Ca2+ influx through TRPC6 accelerate wound closure in keratinocytes, Takada, H;Furuya, K;Sokabe, M;,
J. Cell. Sci. Aug 2014
[TRPC6] Direct Transcriptional Repression of Zfp423 by Zfp521 Mediates a Bone Morphogenic Protein-Dependent Osteoblast versus Adipocyte Lineage Commitment Switch, Addison, WN;Fu, MM;Yang, HX;Lin, Z;Nagano, K;Gori, F;Baron, R;,
Mol. Cell. Biol. Aug 2014
[Zfp521] Direct Transcriptional Repression of Zfp423 by Zfp521 Mediates a Bone Morphogenic Protein-Dependent Osteoblast versus Adipocyte Lineage Commitment Switch, Addison, WN;Fu, MM;Yang, HX;Lin, Z;Nagano, K;Gori, F;Baron, R;,
Mol. Cell. Biol. Aug 2014
 Sphingomyelin Synthase 1 Regulates Neuro-2a Cell Proliferation and Cell Cycle Progression Through Modulation of p27 Expression and Akt Signaling, Wesley, UV;Hatcher, JF;Dempsey, RJ;,
Mol. Neurobiol. Aug 2014
 Identification of a novel AMPK-PEA15 axis in the anoikis-resistant growth of mammary cells, Hindupur, SK;Balaji, SA;Saxena, M;Pandey, S;Sravan, G;Heda, N;Kumar, M;Mukherjee, G;Dey, D;Rangarajan, A;,
Breast Cancer Res. Aug 2014
[PRKAA2] Amplified lipid rafts of malignant cells constitute a target for inhibition of aberrantly active NFAT and melanoma tumor growth by the aminobisphosphonate zoledronic acid, Levin Gromiko, U;Koshelev, V;Kushnir, P;Fedida-Metula, S;Voronov, E;Fishman, D;,
Carcinogenesis Aug 2014
[STIM1] Transcriptional Regulation of STAT3 by SPTBN1 and SMAD3 in HCC Through cAMP-Response Element-Binding Proteins ATF3 and CREB2*, Lin, L;Yao, Z;Bhuvaneshwar, K;Gusev, Y;Kallakury, B;Yang, S;Shetty, K;He, AR;,
Carcinogenesis Aug 2014
[SPTBN1] Phosphorylation of Cdc42 Effector Protein-4 (CEP4) by Protein Kinase C Promotes Motility of Human Breast Cells, Zhao, X;Rotenberg, SA;,
J. Biol. Chem. Aug 2014
[ARHGEF17] Notch3 is necessary for neuronal differentiation and maturation in the adult spinal cord, Rusanescu, G;Mao, J;,
J. Cell. Mol. Med. Aug 2014
[Notch3] Wood Smoke Enhances Cigarette Smoke-Induced Inflammation by Inducing the Aryl Hydrocarbon Receptor Repressor in Airway Epithelial Cells, Awji, EG;Chand, H;Bruse, S;Smith, KR;Colby, JK;Mebratu, Y;Levy, BD;Tesfaigzi, Y;,
Am. J. Respir. Cell Mol. Biol. Aug 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping