These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
The use of this shRNA has been cited in the following citations:
Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK, Adel I. Aziziyeh, Timothy T. Li, Cynthia Pape, Macarena Pampillo, Peter Chidiac, Fred Possmayer,
Andy V. Babwah, Moshmi Bhattacharya,
Cellular Signalling, Volume 21, Issue 7, July 2009, Pages 1207-1217
[RALb] ß-Arrestin/Ral Signaling Regulates Lysophosphatidic Acid–Mediated Migration and Invasion of Human Breast Tumor Cells, Timothy T. Li, Mistre Alemayehu, Adel I. Aziziyeh, Cynthia Pape, Macarena Pampillo, Lynne-Marie Postovit, Gordon B. Mills, Andy V. Babwah, and Moshmi Bhattacharya,
Mol. Cancer Res., Jul 2009; 7: 1064 - 1077
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping