Summary: The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
The use of this shRNA has been cited in the following citations:
BMK1 Kinase Suppresses Epithelial–Mesenchymal Transition through the Akt/GSK3ß Signaling Pathway, Runqiang Chen, Qingkai Yang, and Jiing-Dwan Lee,
Cancer Res., Mar 2012; 72: 1579 - 1587.
[SNAI1] Proinflammatory Mediators Upregulate Snail in Head and Neck Squamous Cell Carcinoma, Maie A. St. John, Mariam Dohadwala, Jie Luo, Guanyu Wang, Gina Lee, Hubert Shih, Eileen Heinrich, Kostantyn Krysan, Tonya Walser, Saswati Hazra, Li Zhu, Chi Lai, Elliot Abemayor, Michael Fishbein, David A. Elashoff, Sherven Sharma, and Steven M. Dubinett,
Clin. Cancer Res., Oct 2009; 15: 6018 - 6027
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping