Summary: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Mitochondrial damage and cholesterol storage in human hepatocellular carcinoma cells with silencing of UBIAD1 gene expression, Morales, CR;Grigoryeva, LS;Pan, X;Bruno, L;,
Molecular Genetics and Metabolism Reports Sep 2014
[UBIAD1] Transmembrane Domain Targeting Peptide Antagonizing ErbB2/Neu Inhibits Breast Tumor Growth and Metastasis, Arpel, A;Sawma, P;Spenlé, C;Fritz, J;Meyer, L;Garnier, N;Velázquez-Quesada, I;Hussenet, T;Aci-Sèche, S;Baumlin, N;Genest, M;Brasse, D;Hubert, P;Crémel, G;Orend, G;Laquerrière, P;Bagnard, D;,
Cell Rep Sep 2014
[ERBB2] PGC-1a mediates mitochondrial biogenesis and oxidative phosphorylation in cancer cells to promote metastasis, LeBleu, VS;O'Connell, JT;Gonzalez Herrera, KN;Wikman, H;Pantel, K;Haigis, MC;de Carvalho, FM;Damascena, A;Domingos Chinen, LT;Rocha, RM;Asara, JM;Kalluri, R;,
Nat. Cell Biol. Sep 2014
[PPARGC1A] Leukocyte specific protein-1: A novel regulator of hepatocellular proliferation and migration deleted in human HCC, Koral, K;Paranjpe, S;Bowen, WC;Mars, W;Luo, J;Michalopoulos, GK;,
Hepatology Sep 2014
[ LSP1] Inhibition of tumor-associated avß3 integrin regulates the angiogenic switch by enhancing expression of IGFBP-4 leading to reduced melanoma growth and angiogenesis in vivo, Contois, LW;Akalu, A;Caron, JM;Tweedie, E;Cretu, A;Henderson, T;Liaw, L;Friesel, R;Vary, C;Brooks, PC;,
Angiogenesis Sep 2014
[ITG?3] Pannexin 1 and Pannexin 3 Channels Regulate Skeletal Muscle Myoblast Proliferation and Differentiation, Langlois, S;Xiang, X;Young, K;Cowan, BJ;Penuela, S;Cowan, KN;,
J. Biol. Chem. Sep 2014
[Panx3] TGF-ß1 mediates the radiation response of prostate cancer, Wu, CT;Hsieh, CC;Yen, TC;Chen, WC;Chen, MF;,
J. Mol. Med. Sep 2014
[TGFB1] Beclin-1-p53 interaction is crucial for cell fate determination in embryonal carcinoma cells, Tripathi, R;Ash, D;Shaha, C;,
J. Cell. Mol. Med. Sep 2014
[ATG5] Beclin-1-p53 interaction is crucial for cell fate determination in embryonal carcinoma cells, Tripathi, R;Ash, D;Shaha, C;,
J. Cell. Mol. Med. Sep 2014
[BECN1] Bile Acids Regulate Nuclear Receptor (Nur77) Expression and Intracellular Location to Control Proliferation and Apoptosis, Hu, Y;Chau, T;Liu, HX;Liao, D;Keane, R;Nie, Y;Yang, H;Wan, YJ;,
Mol. Cancer Res. Sep 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping