Summary: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma, Ye, CS;Zhou, DN;Yang, QQ;Deng, YF;,
Int J Clin Exp Pathol Apr 2014
[SATB1] ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism, Yang, Y;Ahn, YH;Chen, Y;Tan, X;Guo, L;Gibbons, DL;Ungewiss, C;Peng, DH;Liu, X;Lin, SH;Thilaganathan, N;Wistuba, II;Rodriguez-Canales, J;McLendon, G;Creighton, CJ;Kurie, JM;,
J. Clin. Invest. April 2014
[FOG2] HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation, Hu, B;Li, S;Zhang, X;Zheng, X;,
Nucleic Acids Res. April 2014
[USP7] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD4] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD2] Culture dimensionality influences the resistance of glioblastoma stem-like cells to multikinase inhibitors, Fernandez-Fuente, G;Mollinedo, P;Grande, L;Vazquez-Barquero, A;Fernandez-Luna, JL;,
Mol. Cancer Ther. April 2014
[PDGFRA] HSV-2 Increases TLR4-Dependent Phosphorylated IRFs and IFN-ß Induction in Cervical Epithelial Cells, Liu, H;Chen, K;Feng, W;Guo, J;Li, H;,
PLoS ONE e94806 9 4. April 2014
[TLR4] Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog, Zheng, J;Wei, CC;Hase, N;Shi, K;Killingsworth, CR;Litovsky, SH;Powell, PC;Kobayashi, T;Ferrario, CM;Rab, A;Aban, I;Collawn, JF;Dell'italia, LJ;,
PLoS ONE e94732 9 4. April 2014
[NR4A1] Primary cilium regulates CaV1.2 expression through Wnt signaling, Muntean, BS;Jin, X;Williams, FE;Nauli, SM;,
J. Cell. Physiol. April 2014
[CACNA1C] A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect, Lopez-Serra, P;Marcilla, M;Villanueva, A;Ramos-Fernandez, A;Palau, A;Leal, L;Wahi, JE;Setien-Baranda, F;Szczesna, K;Moutinho, C;Martinez-Cardus, A;Heyn, H;Sandoval, J;Puertas, S;Vidal, A;Sanjuan, X;Martinez-Balibrea, E;Viñals, F;Perales, JC;Bramsem, JB;Ørntoft, TF;Andersen, CL;Tabernero, J;McDermott, U;Boxer, MB;Heiden, MG;Albar, JP;Esteller, M;,
Nat Commun 3608 5 . April 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping