Summary: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Mechanosensitive ATP release from hemichannels and Ca2+ influx through TRPC6 accelerate wound closure in keratinocytes, Takada, H;Furuya, K;Sokabe, M;,
J. Cell. Sci. Aug 2014
[TRPC6] Direct Transcriptional Repression of Zfp423 by Zfp521 Mediates a Bone Morphogenic Protein-Dependent Osteoblast versus Adipocyte Lineage Commitment Switch, Addison, WN;Fu, MM;Yang, HX;Lin, Z;Nagano, K;Gori, F;Baron, R;,
Mol. Cell. Biol. Aug 2014
[Zfp521] Direct Transcriptional Repression of Zfp423 by Zfp521 Mediates a Bone Morphogenic Protein-Dependent Osteoblast versus Adipocyte Lineage Commitment Switch, Addison, WN;Fu, MM;Yang, HX;Lin, Z;Nagano, K;Gori, F;Baron, R;,
Mol. Cell. Biol. Aug 2014
 Sphingomyelin Synthase 1 Regulates Neuro-2a Cell Proliferation and Cell Cycle Progression Through Modulation of p27 Expression and Akt Signaling, Wesley, UV;Hatcher, JF;Dempsey, RJ;,
Mol. Neurobiol. Aug 2014
 Identification of a novel AMPK-PEA15 axis in the anoikis-resistant growth of mammary cells, Hindupur, SK;Balaji, SA;Saxena, M;Pandey, S;Sravan, G;Heda, N;Kumar, M;Mukherjee, G;Dey, D;Rangarajan, A;,
Breast Cancer Res. Aug 2014
[PRKAA2] Amplified lipid rafts of malignant cells constitute a target for inhibition of aberrantly active NFAT and melanoma tumor growth by the aminobisphosphonate zoledronic acid, Levin Gromiko, U;Koshelev, V;Kushnir, P;Fedida-Metula, S;Voronov, E;Fishman, D;,
Carcinogenesis Aug 2014
[STIM1] Transcriptional Regulation of STAT3 by SPTBN1 and SMAD3 in HCC Through cAMP-Response Element-Binding Proteins ATF3 and CREB2*, Lin, L;Yao, Z;Bhuvaneshwar, K;Gusev, Y;Kallakury, B;Yang, S;Shetty, K;He, AR;,
Carcinogenesis Aug 2014
[SPTBN1] Phosphorylation of Cdc42 Effector Protein-4 (CEP4) by Protein Kinase C Promotes Motility of Human Breast Cells, Zhao, X;Rotenberg, SA;,
J. Biol. Chem. Aug 2014
[ARHGEF17] Notch3 is necessary for neuronal differentiation and maturation in the adult spinal cord, Rusanescu, G;Mao, J;,
J. Cell. Mol. Med. Aug 2014
[Notch3] Wood Smoke Enhances Cigarette Smoke-Induced Inflammation by Inducing the Aryl Hydrocarbon Receptor Repressor in Airway Epithelial Cells, Awji, EG;Chand, H;Bruse, S;Smith, KR;Colby, JK;Mebratu, Y;Levy, BD;Tesfaigzi, Y;,
Am. J. Respir. Cell Mol. Biol. Aug 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping