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Home shRNA All ARHGDIA shRNA/siRNA

ARHGDIA (Gene ID 396) Human shRNA

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Specifications Citations Related Products Product Documents
SKU Description Vector Price Availability*  
TF314680
  • ARHGDIA - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector (Gene ID = 396). 5µg purified plasmid DNA per construct
  • Non-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.



650 2 Weeks
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Also for ARHGDIA (Locus ID 396)
cDNA Clone qPCR Master Mix Primer Pair siRNA Antibody Service
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Reference Data
RefSeq: NM_001185077NM_001185078NM_001301240NM_001301241NM_001301242NM_001301243NM_004309
NR_125441
Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
Summary: This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].
shRNA Design:
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

 


* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping

 

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