Summary: Humans lipodystrophy is characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mice carrying mutations in the fatty liver dystrophy (fld) gene have similar phenotypes. Through positional cloning, the mouse gene responsible for fatty liver dystrophy was isolated and designated Lpin1. The nuclear protein encoded by Lpin1 was named lipin. Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of preadipocytes. These results indicated that lipin is required for normal adipose tissue development and provided a candidate gene for human lipodystrophy. Through database searches, mouse and human EST and genomic sequences with similarities to Lpin1 were identified. These included two related mouse genes (Lpin2 and Lpin3) and three human homologs (LPIN1, LPIN2, and LPIN3). Human LPIN1 gene has been mapped to 2p25.; linkages of fat mass and serum leptin levels to this same region have been noted. Human LPIN2 and LPIN3 mapped to chromosomes 18p11 and 20q11-q12, respectively. The mouse genes encoding Lpin1, Lpin2, and Lpin3 mapped to chromosome 12, 17, and 2, respectively. [provided by RefSeq, Jul 2008]. COMPLETENESS: complete on the 3' end.
shRNA Design:
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
shRNA Citations:
The SNARE Proteins SNAP25 and Synaptobrevin Are Involved in Endocytosis at Hippocampal Synapses, Zhen Zhang, Dongsheng Wang, Tao Sun, Jianhua Xu, Hsueh-Cheng Chiang, Wonchul Shin, and Ling-Gang Wu,
J. Neurosci., May 2013; 33: 9169 - 9175.
[SNAP25 ] The SNARE Proteins SNAP25 and Synaptobrevin Are Involved in Endocytosis at Hippocampal Synapses, Zhen Zhang, Dongsheng Wang, Tao Sun, Jianhua Xu, Hsueh-Cheng Chiang, Wonchul Shin, and Ling-Gang Wu,
J. Neurosci., May 2013; 33: 9169 - 9175.
[VAMP2 ] Mitochondrial transcription factor A regulated ionizing radiation-induced mitochondrial biogenesis in human lung adenocarcinoma A549 cells, Jing Yu, Qisen Wang, Ni Chen, Yuxiang Sun, Xiaofei Wang, Lijun Wu, Shaopeng Chen, Hang Yuan, An Xu, and Jun Wang,
J Radiat Res, May 2013; 10.1093/jrr/rrt046.
[TFAM] Inhibition of prolyl 4-hydroxylase, beta polypeptide (P4HB) attenuates temozolomide resistance in malignant glioma via the endoplasmic reticulum stress response (ERSR) pathways, Stella Sun, Derek Lee, Amy S.W. Ho, Jenny K.S. Pu, X.Q. Zhang, Nikki P. Lee, Philip J.R. Day, W.M. Lui, C.F. Fung, and Gilberto K.K. Leung,
Neuro Oncology, May 2013; 15: 562 - 577.
[P4HB] Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic lung disease, Yi Jia, Joanne Domenico, Katsuyuki Takeda, Junyan Han, Meiqin Wang, Michael Armstrong, Nichole Reisdorph, Brian P. O’Connor, Joseph J. Lucas, and Erwin W. Gelfand,
PNAS, May 2013; 110: 8152 - 8157.
[Cyp11a1 ] miR-26a Plays an Important Role in Cell Cycle Regulation in ACTH-Secreting Pituitary Adenomas by Modulating Protein Kinase C, Erica Gentilin, Federico Tagliati, Carlo Filieri, Daniela Molè, Mariella Minoia, Maria Rosaria Ambrosio, Ettore C degli Uberti, and Maria Chiara Zatelli,
Endocrinology, May 2013; 154: 1690 - 1700.
[PRKCD] BAF complexes facilitate decatenation of DNA by topoisomerase IIa, Emily C. Dykhuizen, Diana C. Hargreaves, Erik L. Miller, Kairong Cui, Andrey Korshunov, + et al.,
Nature 497, 624-627 doi:10.1038/nature12146
[Arid1a ] Deacetylation of p53 induces autophagy by suppressing Bmf expression, Amelia U. Contreras, Yohannes Mebratu, Monica Delgado, Gilbert Montano, Chien-an A. Hu, Stefan W. Ryter, Augustine M.K. Choi, Yuting Lin, Jialing Xiang, Hitendra Chand, and Yohannes Tesfaigzi,
J. Cell Biol., Apr 2013; 201: 427 - 437.
[p53] Premetastatic soil and prevention of breast cancer brain metastasis, Yan Liu, Akemi Kosaka, Maki Ikeura, Gary Kohanbash, Wendy Fellows-Mayle, Linda A. Snyder, and Hideho Okada,
Neuro Oncology, Apr 2013; 10.1093/neuonc/not031.
[COX2] Altered localization, abnormal modification and loss of function of Sigma receptor-1 in amyotrophic lateral sclerosis, J. Prause, A. Goswami, I. Katona, A. Roos, M. Schnizler, E. Bushuven, A. Dreier, S. Buchkremer, S. Johann, C. Beyer, M. Deschauer, D. Troost, and J. Weis,
Hum. Mol. Genet., Apr 2013; 22: 1581 - 1600.
[SIGMAR1]
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping
