OriGene - RNAi - HuSH Collection

OriGene shRNA Recent Citations

Annexin A2 is a C-terminal PCSK9 binding protein that regulates endogenous LDL receptor levels, J. Biol. Chem., Sep 2008; 10.1074/jbc.M805971200. [ANAX2]

Cytoglobin, the Newest Member of the Globin Family, Functions as a Tumor Suppressor Gene, Cancer Res., Sep 2008; 68: 7448 - 7456 [CYGB]

Identification of a Small Molecule with Synthetic Lethality for K-Ras and Protein Kinase C Iota, Cancer Res. 2008; 68(18): p. 7403-7408 [PRKCI, PRKCZ]

Polyethylene glycol-mediated colorectal cancer chemoprevention: roles of epidermal growth factor receptor and Snail, Mol. Cancer Ther., Sep 2008; 7: 3103 - 3111 [EGFR]

Separate cis-trans Pathways Post-transcriptionally Regulate Murine CD154 (CD40 Ligand) Expression: A NOVEL FUNCTION FOR CA REPEATS IN THE 3'-UNTRANSLATED REGION, J. Biol. Chem., Sep 2008; 283: 25606 - 25616 [HNRNPL]

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HuSH-29: Pre-designed shRNA

HuSH-29 are pre-designed shRNA with genome wide coverage (human and mouse). It takes advantage of the superiority of 29mer shRNA and delivers guaranteed knock-down.
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Features and Benefits

Two retroviral vector options

5ug of purified plasmid for immediate transfection

Guaranteed successful gene knockdown

Versatile applications: transient or stable transfection or retroviral infection

$595 for a kit (4 unique gene-specific shRNA plasmids and two negative control plasmids)

Applications:

Gene silencing through transient or stable transfection

Gene silencing through retroviral transduction (see protocol)

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