Identification of CTCF as a master regulator of the clustered protocadherin genes
Nucleic Acids Res., Apr 2012; 40: 3378 - 3391.
[CTCF
]
Transducin-like Enhancer of Split-1 (TLE1) Combines with Forkhead Box Protein G1 (FoxG1) to Promote Neuronal Survival
J. Biol. Chem., Apr 2012; 287: 14749 - 14759.
[Tle1
]
Motoneurons Secrete Angiogenin to Induce RNA Cleavage in Astroglia
J. Neurosci., Apr 2012; 32: 5024 - 5038.
[GFP-tagged siRNA]
CCR7/CCL19 Controls Expression of EDG-1 in T Cells
J. Biol. Chem., Apr 2012; 287: 11656 - 11664.
[CCL19
]
Examples of Downregulation of Other Gene Overexpression by HuSH Constructs
HEK293T cells were plated at 2 X 105 ml for 2ml into a 6-well plate one day before transfection. One ug of each shRNA construct was cotransfected with 300 ng of the corresponding cDNA plasmid using 4 ul TurboFectin 8.0 for transient transfection. Cells were lysed 72 hrs post transfection . Five ug protein were loaded on Invitrogen NuPage Gel system and separated proteins were immunoblotted with corresponding Validated Antibodies from OriGene.
