OriGene - shRNA

OriGene shRNA Recent Citations

Annexin A2 is a C-terminal PCSK9 binding protein that regulates endogenous LDL receptor levels, J. Biol. Chem., Sep 2008; 10.1074/jbc.M805971200. [ANAX2]

Cytoglobin, the Newest Member of the Globin Family, Functions as a Tumor Suppressor Gene, Cancer Res., Sep 2008; 68: 7448 - 7456 [CYGB]

Identification of a Small Molecule with Synthetic Lethality for K-Ras and Protein Kinase C Iota, Cancer Res. 2008; 68(18): p. 7403-7408 [PRKCI, PRKCZ]

Polyethylene glycol-mediated colorectal cancer chemoprevention: roles of epidermal growth factor receptor and Snail, Mol. Cancer Ther., Sep 2008; 7: 3103 - 3111 [EGFR]

Separate cis-trans Pathways Post-transcriptionally Regulate Murine CD154 (CD40 Ligand) Expression: A NOVEL FUNCTION FOR CA REPEATS IN THE 3'-UNTRANSLATED REGION, J. Biol. Chem., Sep 2008; 283: 25606 - 25616 [HNRNPL]

All Citations
click here

Downregulation of HIF1A Expression by HuSH Constructs

HEK293T cells were plated at 2 X 10⁵per ml (2ml) into a 6-well plate one day before transfection. One (1) ug Hush plasmid was used together with 4 ul TurboFectin 8.0 for transient transfection as suggested by OriGene transfection protocol in the presence of 300 ng SC119189 encoding HIF1A. Seventy two (72) hrs post transfection cells were lysed. Five (5) ug protein were loaded on Invitrogen NuPage Gel system and separated proteins were immunoblotted with anti-HIF1A Rabbit mAb (TA300045) and reblotted with anti-Erk1/2 (Santa Cruz Biotechnlogy) Abs.