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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Molecular Tools
PrecisionShuttle ORF Vector System

OriGene cDNA clones in recent publications
A coding-independent function of an alternative Ube3a transcript during neuronal development Nat. Neurosci. May 2015 [Ube3a]

Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation by inhibiting angiotensin-(1-7)/Mas signaling Oncol. Rep. May 2015 [MAS1]

Suppressed expression of LDHB promotes pancreatic cancer progression via inducing glycolytic phenotype Med. Oncol. May 2015 [LDHB]

The novel S59P mutation in the TNFRSF1A gene identified in an adult onset TNF receptor associated periodic syndrome (TRAPS) constitutively activates NF-?B pathway Arthritis Res. Ther. May 2015 [TNFRSF1A]

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The Rapid-Screen™ Arrayed cDNA Library system utilizes two separate plates - a "Master Plate" and a "Sub- Plate" - to isolate the clone of interest. The Master Plate is a standard 96-well microtiter plate arrayed with a size-selected cDNA library at a titer of approximately 5000 clones per well. The Master Plate is screened using gene-specific primers followed by agarose electrophoresis of the PCR products.

Depending on the abundance of the gene of interest, positive wells are subsequently identified via corresponding bands on agarose gel. For each well of the Master Plate there is a corresponding Sub-Plate. Each Sub-Plate is arrayed at a titer of 50 clones per well. Gene-specific PCR is performed on the Sub-Plate to identify positive wells. Sub-plate clones are housed in E.coli. A small aliquot from the positive well(s) of the Sub-Plate are plated onto standard LB agar and grown overnight. Using colony PCR, 96-colonies are screened with an average of approximately two positive clones per positive Sub-Plate well. These clones are easily amplified for further analysis.

Kit Components

  • 96-well arrayed cDNA library Master Plate (5000 clones/well)
  • Rapid-Screen vector primer pCMV6-XL4 (10 pmol/?l)
  • Rapid-Screen positive control (483 bp)
  • Quanti-Ladder™ DNA Marker - a ready-to-use molecular marker for easy quantitation and fragment size determination (100 ?l for 20 lanes)
  • Four 96-well plate re-sealing tapes
  • 96-well arrayed cDNA library Sub-Plate (50 clones/well).
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