DESCRIPTION

Rapid-Screen™ Arrayed cDNA Library quality is improved by first isolating double-stranded cDNAs of different size-fractions and then ligating them separately into the vector. The cloning process has been made faster and more economical by having the libraries arrayed in 96-well plates and developing a screening procedure that is PCR based. Since the screening is PCR-based, vector and gene-specific primers can be used in concert to determine the size of the various cDNA inserts after the very first PCR screen, thus eliminating the need to isolate many clones and then find out which, if any, are full-length.