OriGene - Reagents & Tools - Rapid-Screen Arrayed cDNA Library Panels

OriGene cDNAs cited in recent published Functional Studies

Human apolipoprotein E is required for Infectivity and Production of Hepatitis C Virus in Cell Culture [APOE] J. Virol. 2007 Dec;81(24):13783-93

The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity [NET1]
Mol Cell Biol. 2007 Dec;27(24):8683-97

Differential expression of novel tyrosine kinase substrates during breast cancer development [NSFL1C]
Mol Cell Proteomics. 2007 Dec;6(12):2072-87

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DESCRIPTION

Rapid-Screen™ Arrayed cDNA Library quality is improved by first isolating double-stranded cDNAs of different size-fractions and then ligating them separately into the vector. The cloning process has been made faster and more economical by having the libraries arrayed in 96-well plates and developing a screening procedure that is PCR based. Since the screening is PCR-based, vector and gene-specific primers can be used in concert to determine the size of the various cDNA inserts after the very first PCR screen, thus eliminating the need to isolate many clones and then find out which, if any, are full-length.