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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
Description
Format
Application
cDNA Libraries
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
C5aR is frequently expressed in metastatic renal cell carcinoma and plays a crucial role in cell invasion via the ERK and PI3 kinase pathways Oncol. Rep. Apr 2015 [C5ar1]

MAC-T Cells as a Tool to Evaluate Lentiviral Vector Construction Targeting Recombinant Protein Expression in Milk Anim. Biotechnol. Apr 2015 [IL2]

A Novel Technology for Multiplex Gene Expression Analysis Directly from Whole Blood Samples Stabilized at Ambient Temperature Using an RNA-Stabilizing Buffer J Mol Diagn Mar 2015 [PCNA]

GATA2 Germline Mutations Impair GATA2 Transcription, Causing Haploinsufficiency: Functional Analysis of the p.Arg396Gln Mutation J. Immunol. Mar 2015 [GATA2]

View All Citations >>

APPLICATION

Rapid-Screen™ Arrayed cDNA Libraries can be used to clone alternatively spliced transcripts, even if the desired spliced variant is many times less abundant than its parent transcript. The detection of different-sized fragments through use of the 5' vector primer plus the 3' gene-specific primer is sometimes attributable to alternative-splicing rather than to truncated transcripts. If the alternate splicing has already been characterized, gene-specific primers that differentiate between the two spliced forms can be designed.

Rapid-Screen Arrayed cDNA Libraries were used with a pair of gene-specific primers to demonstrate a pooling technique for the detection of two alternatively spliced transcripts of a gene. The shorter form was much more abundant than the longer one. The shorter appeared in seven of the twelve column pools (40,000 clones/pool) and four of the eight row pools (60,000 clones/pool). The longer form (approximately 210 bp longer) was found only in the column 1 pool and in the row H pool. Thus well H1 of the Master Plate contains the lower abundant transcript variant.

RFAL Fig 1

 

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