OriGene Technologies, Inc.
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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
Description
Format
Application
cDNA Libraries
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
Early Changes in Cytochrome P450s and Their Associated Arachidonic Acid Metabolites Play a Crucial Role in the Initiation of Cardiac Hypertrophy Induced by Isoproterenol Drug Metab. Dispos. Aug 2015 [CYP2J2]

Theaflavin-3,3'-digallate, a black tea polyphenol, stimulates lipolysis associated with the induction of mitochondrial uncoupling proteins and AMPK–FoxO3A–MnSOD pathway in 3T3-L1 adipocytes Journal of Functional Foods Aug 2015 [SOD2]

NFkB is activated by multiple mechanisms in hairy cell leukemia Genes Chromosomes Cancer Jul 2015 [PITX1]

Systematic investigation of CMTM family genes suggests relevance to glioblastoma pathogenesis and CMTM1 and CMTM3 as priority targets Genes Chromosomes Cancer Jul 2015 [CMTM2]

View All Citations >>

APPLICATION

Rapid-Screen™ Arrayed cDNA Libraries can be used to clone alternatively spliced transcripts, even if the desired spliced variant is many times less abundant than its parent transcript. The detection of different-sized fragments through use of the 5' vector primer plus the 3' gene-specific primer is sometimes attributable to alternative-splicing rather than to truncated transcripts. If the alternate splicing has already been characterized, gene-specific primers that differentiate between the two spliced forms can be designed.

Rapid-Screen Arrayed cDNA Libraries were used with a pair of gene-specific primers to demonstrate a pooling technique for the detection of two alternatively spliced transcripts of a gene. The shorter form was much more abundant than the longer one. The shorter appeared in seven of the twelve column pools (40,000 clones/pool) and four of the eight row pools (60,000 clones/pool). The longer form (approximately 210 bp longer) was found only in the column 1 pool and in the row H pool. Thus well H1 of the Master Plate contains the lower abundant transcript variant.

RFAL Fig 1

 

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