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OriGene cDNA clones in recent publications
Regulator of G protein signalling 14 attenuates cardiac remodelling through the MEK-ERK1/2 signalling pathway Basic Res. Cardiol. Jul 2016 [Rgs14]

Alpha-synuclein modulates retinal iron homeostasis by facilitating the uptake of transferrin-bound iron: Implications for visual manifestations of Parkinson's disease Free Radic. Biol. Med. Jun 2016 [SNCA]

Apoptosis as the focus of an authentic research experience in a cell physiology laboratory Adv Physiol Educ Jun 2016 [BCL2]

Arylsulfatase B mediates the sulfonation-transport interplay in human embryonic kidney (HEK) 293 cells overexpressing sulfotransferase 1A3 Drug Metab. Dispos. Jun 2016 [STS]

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RAPID-LOAD PCR LOADING DYE

Rapid-Load PCR Loading Dye is a 5x sample loading dye that is added to a PCR mix before amplification. The presence of Rapid-Load in the PCR mix will not interfere with the performance of the overall reaction. After the PCR is finished, the reaction products can be directly loaded in an agarose gel with no addition of standard loading dye necessary. This product can be used in PCR amplifications containing standard plasmid DNA, genomic DNA or bacterial colonies. It is also ideal for use with multiple samples such as when performing a 96-well PCR.

  • No PCR reaction interference
  • Load PCR samples directly on gel
  • Ideal for 96-well PCR

Rapid-Load PCR Loading Dye is red in color making it easy to load on agarose gels and track during electrophoresis. The red dye migrates at 1.4 kbp in a 1% agarose gel with 1x TAE running buffer. Fig.1 shows PCR amplification of human ?-actin from plasmid DNA, transformed bacterial colonies, and genomic DNA. Each reaction was performed in duplicate in the presence (+) or absence (-) of Rapid-Load.

RL Fig 1

Fig.1

 

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