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OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017 [GRIN2A]

Engineering brown fat into skeletal muscle using ultrasound-targeted microbubble destruction gene delivery in obese Zucker rats: Proof of concept design IUBMB Life 2017 [BMP7]

Traditional Chinese medicine, Fuzheng Kang‑Ai decoction, inhibits metastasis of lung cancer cells through the STAT3/MMP9 pathway Mol Med Rep 2017 [PIAS3]

A Novel Egr-1-Agrin Pathway and Potential Implications for Regulation of Synaptic Physiology and Homeostasis at the Neuromuscular Junction Front Aging Neurosci 2017 [EGR1]

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RAPID-LOAD PCR LOADING DYE

Rapid-Load PCR Loading Dye is a 5x sample loading dye that is added to a PCR mix before amplification. The presence of Rapid-Load in the PCR mix will not interfere with the performance of the overall reaction. After the PCR is finished, the reaction products can be directly loaded in an agarose gel with no addition of standard loading dye necessary. This product can be used in PCR amplifications containing standard plasmid DNA, genomic DNA or bacterial colonies. It is also ideal for use with multiple samples such as when performing a 96-well PCR.

  • No PCR reaction interference
  • Load PCR samples directly on gel
  • Ideal for 96-well PCR

Rapid-Load PCR Loading Dye is red in color making it easy to load on agarose gels and track during electrophoresis. The red dye migrates at 1.4 kbp in a 1% agarose gel with 1x TAE running buffer. Fig.1 shows PCR amplification of human ?-actin from plasmid DNA, transformed bacterial colonies, and genomic DNA. Each reaction was performed in duplicate in the presence (+) or absence (-) of Rapid-Load.

RL Fig 1

Fig.1

 

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