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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Molecular Tools
Millenium™ RNA Marker
Quanti-Ladder™ DNA Marker
Rapid-Load™ PCR Loading Dye
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
A coding-independent function of an alternative Ube3a transcript during neuronal development Nat. Neurosci. May 2015 [Ube3a]

Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation by inhibiting angiotensin-(1-7)/Mas signaling Oncol. Rep. May 2015 [MAS1]

Suppressed expression of LDHB promotes pancreatic cancer progression via inducing glycolytic phenotype Med. Oncol. May 2015 [LDHB]

The novel S59P mutation in the TNFRSF1A gene identified in an adult onset TNF receptor associated periodic syndrome (TRAPS) constitutively activates NF-?B pathway Arthritis Res. Ther. May 2015 [TNFRSF1A]

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RAPID-LOAD PCR LOADING DYE

Rapid-Load PCR Loading Dye is a 5x sample loading dye that is added to a PCR mix before amplification. The presence of Rapid-Load in the PCR mix will not interfere with the performance of the overall reaction. After the PCR is finished, the reaction products can be directly loaded in an agarose gel with no addition of standard loading dye necessary. This product can be used in PCR amplifications containing standard plasmid DNA, genomic DNA or bacterial colonies. It is also ideal for use with multiple samples such as when performing a 96-well PCR.

  • No PCR reaction interference
  • Load PCR samples directly on gel
  • Ideal for 96-well PCR

Rapid-Load PCR Loading Dye is red in color making it easy to load on agarose gels and track during electrophoresis. The red dye migrates at 1.4 kbp in a 1% agarose gel with 1x TAE running buffer. Fig.1 shows PCR amplification of human ?-actin from plasmid DNA, transformed bacterial colonies, and genomic DNA. Each reaction was performed in duplicate in the presence (+) or absence (-) of Rapid-Load.

RL Fig 1

Fig.1

 

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