OriGene Technologies, Inc.
Register | Shopping Cart | Blast your Gene | Home
Search:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Other Products Tech Support
orange
Blue-Ribbon Poly A+ RNA and Total RNA
Sure-Race CDNA 5' End Discovery Panels
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNAs cited in recent published Functional Studies

Human apolipoprotein E is required for Infectivity and Production of Hepatitis C Virus in Cell Culture [APOE] J. Virol. 2007 Dec;81(24):13783-93

The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity [NET1]
Mol Cell Biol. 2007 Dec;27(24):8683-97

Differential expression of novel tyrosine kinase substrates during breast cancer development [NSFL1C]
Mol Cell Proteomics. 2007 Dec;6(12):2072-87

More Citations
click here

 

APPLICATION

  • The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
  • Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
  • The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
  • The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

 


Copyright © 2008 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
6 Taft Court • Suite 100 • Rockville, MD 20850 • 1.888.267.4436