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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
GFP-tagged Apolipoprotein E: a useful marker for the study of hepatic lipoprotein egress Traffic 2017 [CANX]

Aldehyde dehydrogenase activity plays no functional role in stem cell-like properties in anaplastic thyroid cancer cell lines Endocrine 2016 [ALDH1A3]

Asymmetric binding and metabolism of polyunsaturated fatty acids (PUFAs) by CYP2J2 epoxygenase Biochemistry 2016 [CYP2J2]

Copy number variation as a genetic basis for heterotaxy and heterotaxy-spectrum congenital heart defects Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2016 [PFKP]

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APPLICATION

  • The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
  • Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
  • The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
  • The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

 

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