OriGene Technologies, Inc.
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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
1,2,4-Triazolyl octahydropyrrolo[2,3-b]pyrroles: A new series of potent and selective dopamine D3 receptor antagonists Bioorg. Med. Chem. Apr 2016 [DRD3]

A role for ABCG2 beyond drug transport: regulation of autophagy Autophagy Mar 2016 [ABCG2]

An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal NATURE COMMUNICATIONS Mar 2016 [U2AF2]

Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL Molecular Therapy nucleic acid Mar 2016 [AXL]

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APPLICATION

  • The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
  • Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
  • The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
  • The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

 

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