OriGene Technologies, Inc.
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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
Citrullinated Vimentin Presented on MHC-II in Tumor Cells Is a Target for CD4+ T-Cell-Mediated Antitumor Immunity Cancer Res. Feb 2016 [MOG]

A short conserved motif in ALYREF directs cap-and EJC-dependent assembly of export complexes on spliced mRNAs Nucleic Acids Research Jan 2016 [ALYREF]

An integrated systems biology approach identifies positive cofactor 4 as a factor that increases reprogramming efficiency Nucleic Acids Res. Jan 2016 [SUB1]

Asc-1 Transporter Regulation of Synaptic Activity via the Tonic Release of d-Serine in the Forebrain Cereb. Cortex Jan 2016 [SLC7A10]

View All Citations >>

APPLICATION

  • The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
  • Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
  • The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
  • The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

 

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