OriGene cDNA clones in recent publications
Activation and Inhibition of Thermosensitive TRP Channels by Voacangine, an Alkaloid Present in Voacanga africana, an African Tree J. Nat. Prod., Feb 2014.
Flavanones from Miconia prasina Phytochemistry Letters, Feb 2014.
Glycosylation of a disintegrin and metalloprotease 17 affects its activity and inhibition Anal. Biochem., Feb 2014.
MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation Biochim. Biophys. Acta, Feb 2014.
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- The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
- Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
- The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
- The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.