OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017
Angiotensin II degrades myeloid cell leukemia 1 in human umbilical vein endothelial cells IUBMB Life 2017
COLEC10 is mutated in 3MC patients and regulates early craniofacial development PLoS Genet 2017
Contrasting roles of the ABCG2 Q141K variant in prostate cancer Exp. Cell Res 2017
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- The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
- Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
- The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
- The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.