OriGene cDNA clones in recent publications
A new monoclonal antibody against human alpha-dystroglycan reveals reduced core protein in some, but not all, dystroglycanopathy patients Neuromuscular Disorders Sep 2014
An extracellular-matrix-specific GEF-GAP interaction regulates Rho GTPase crosstalk for 3D collagen migration Nat. Cell Biol. Sep 2014
Cell cycle restriction is more important than apoptosis induction for RASSF1A tumor suppression J. Biol. Chem. Sep 2014
Distinct Roles of Ape1 Protein, An Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing J. Biol. Chem. Sep 2014
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- The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
- Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
- The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
- The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.