OriGene cDNA clones in recent publications
Nuc-ErbB3 regulates H3K27me3 levels and HMT activity to establish epigenetic repression during peripheral myelination Glia Jun 2016
PKCe inhibits isolation and stemness of side population cells via the suppression of ABCB1 transporter and PI3K/Akt, MAPK/ERK signaling in renal cell carcinoma cell line 769P Cancer Lett. Jun 2016
AIG1 and ADTRP are atypical integral membrane hydrolases that degrade bioactive FAHFAs Nat. Chem. Biol. May 2016
miR-613 inhibits the growth and invasiveness of human hepatocellular carcinoma via targeting DCLK1 Biochem. Biophys. Res. Commun. May 2016
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- The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
- Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
- The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
- The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.