OriGene Technologies, Inc.
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Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early-onset autoinflammatory disease Nat. Genet. Jan 2016 [TRAF6]

Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma Journal of the Neurological Sciences Jan 2016 [POSTN]

The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial-Like Cells Stem Cells International Jan 2016 [HMBOX1]

a-Helix-Peptides Composing the Human Nuclear Receptor ERR? Competitively Provoke Inhibition of Functional Homomeric Dimerization Biopolymers Dec 2015 [AR]

View All Citations >>

APPLICATION

  • The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
  • Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
  • The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
  • The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

 

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