OriGene cDNA clones in recent publications
1,2,4-Triazolyl octahydropyrrolo[2,3-b]pyrroles: A new series of potent and selective dopamine D3 receptor antagonists Bioorg. Med. Chem. Apr 2016
A role for ABCG2 beyond drug transport: regulation of autophagy Autophagy Mar 2016
An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal NATURE COMMUNICATIONS Mar 2016
Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL Molecular Therapy nucleic acid Mar 2016
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- The cDNA libraries were designed as to complement the DupLEX-A yeast two-hybrid system. The cDNA are cloned downstream of B42-HA domain under Gal promoter. For the customer who already have the Duplex-A system in house, the libraries can be used for identification of novel protein interactions with a bait protein.
- Once positive clone is identified, the plasmid can be used to express fusion protein with HA tag which allows detection with HA antibody.
- The library can be used as cDNA template to PCR amplify target genes or gene fragments with specific primer pairs.
- The library can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.