OriGene Technologies, Inc.
Search:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue Other Products Tech Support
orange
Blue-Ribbon Poly A+ RNA and Total RNA
Rapid-Screen Arrayed cDNA Library Panels
cDNA Libraries
Description
Format
Application
Molecular Tools
PrecisionShuttle ORF Vector System


OriGene cDNA clones in recent publications
Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses Cancer Prevention Research, Jan 2012; 5: 122 - 137. [HSPB1 ]

Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells Mol. Cancer Res., Jan 2012; 10: 133 - 142. [SREBF1 ]

Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy Cancer Res., Jan 2012; 72: 527 - 536. [AR ]

CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling J. Biol. Chem., Jan 2012; 287: 1198 - 1209. [Cnr2 ]

View All Citations >>

cDNA LIBRARIES

Along with the DupLEX-A Yeast Two-Hybrid System kit, OriGene offers an extensive list of expression cDNA libraries that are the pivotal component of OriGene's DupLEX-A Yeast Two-Hybrid System kit. For each library, oligo(dT)-primered cDNA was synthesized from high quality mRNAs and cloned unidirectionally between EcoRI and XhoI site of pJG4-5, the activation domain expression vector used in the DupLEX-A system. After plating the primary bacterial transformants, the cells were scraped from the plates, pooled, an aliquot was removed and frozen away and the plasmid was purified from the rest. Thus the researchers need not perform the laborious task of library DNA isolation. Moreover, the provided library DNA has never been amplified.

In addition to the DupLEX-A System kit, we make the libraries available to support the customers who wish to expand their research based upon this methodology.

While designed to complement the yeast two-hybrid system, these libraries can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

Application Guide for DupLEX-A Library          Application Guide for DupLEX-A System        

    Display Products

 

Terms of Use 

Inc 5000 Healthcare Company Copyright © 2012 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436


Reproduction of any materials from this website is strictly forbidden without permission.