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DESCRIPTION
Multiple-Choice™ Total RNA is isolated from fresh or snap-frozen
samples. All mRNAs are extracted from total RNAs using oligo-dT
columns. All polyA+ RNAs are passed through the column twice to
ensure the highest purity and quality evaluated by denaturing agarose
gel and Northern blot hybridization. Sharp ribosomal bands of 28S
and 18S and an approximate 2:1 ratio of 28S rRNA band over 18S ribosomal
band are required to pass product quality control.
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