QUALITY CONTROL

Functionally active proteins are over expressed in and purified from several different systems that have been well characterized.  ProteinOne primarily utilizes bacterial and baculoviral systems to produce proteins with a focus on transcription and cancer related proteins.  The protein purification strategy revolves around utilizing protein tags including 6-histidine, FLAG or myc, to increase purify of the proteins to near homogeneity upon initial purification.  Initial synthesis is the creation of new plasmids containing one or more of the protein tags that do not interfere with the protein function(s).  This is followed by a second step of purification (required to further purify recombinant proteins even with a unique protein tag), which will be decided based on protein structure and property.  These steps involve use of conventional chromatography, fast performance liquid chromatography (FPLC) and / or high-pressure liquid chromatography (HPLC) based techniques to achieve highly purified proteins.  Quality is monitored by SDS-PAGE.

SDS-PAGE of Topo I (C651-765) Human DNA Topoisomerase I (C terminal domain)

ProteinOne proteins are purified greater than 95% homogeneous and contain no detectable protease, DNase, and RNase activity.