PRODUCT APPLICATION

Whereas a human gene transcript supplies the required information to blueprint the protein sequence, in order to test cellular function, it is necessary to produce and test the actual protein.  OriGene has partnered with quality, human protein providers who derive value from OriGenes large transcript repository.  These high-quality protein samples are made accessible through this website. 

Enzymatic Assays:  Protein enzymes can be used to catalyze reactions of interest in search of substrate specificity.  The researcher can also use such assays to assess both activation and inhibition of the enzymes activity.  OriGene provides many quality protein kinases to test their phosphorylation activity.

Protein Binding Studies: Having a properly folded protein is key to determine its biologically relevant interactions.  This type of study is traditionally performed through gel-shift assays, ELISA plate analysis, and various types of affinity chromatography. 

Western Blots and SDS-PAGE:  The identification of a proteins rate of migration (Mr) through SDS-PAGE is best identified through the use of a purified protein.  The proteins available through OriGene are suitable for this purpose.

Functional Studies: In addition to enzymatic or binding assays, the researcher can uncover the cellular function of these proteins through a variety of specialized assays measuring fluctuations in second messengers calcium or camp. Transcriptional regulation can be assessed through off-the-shelf reporter gene assays.