p53 (1-342, C-terminal deletion) Tumor Suppressor Protein and Transcription Factor, 5,000 units

Cat. No.   Description Price TP200118 P2003-01 p53 (1-342, C-terminal deletion) Tumor Suppressor Protein and Transcription Factor, 5,000 units $ 275 The associated gene in an expression vector NM_000546 is also available.

Description:

Human p53 protein is composed of 393 amino acid residues with several distinct regions. In addition to that the N-terminal activation domain allows p53 protein to recruit the basal transcription machinery and activate the expression of target genes, the core domain binds to target DNA in a sequence-specific manner and the majority of mutations found in human tumors occur in the region of the gene encoding this domain, and modification of the C-terminal basic domain regulates p53 function (1-4), the tetramerization domain of p53 plays an important role in cell cycle. Disruption or loss of oligomerization function is associated with loss of cell cycle arrest (5, 6). This mutant protein (with the deletion of C-terminus 51 residues including the entire basic domain and portion of tetramerization domain) can be used as a unique tool to study specific function of p53 related to the C-terminus.

Source:

The C-terminus-deleted p53 (amino acid 1-342) was expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography.

Applications:

Recombinant p53 can be used 1) for gel mobility shift assay or for a DNase I footprinting in the presence of double stranded DNA containing a consensus p53-binding sequence [5’-PuPuPuC(A/T)(T/A)GPyPyPy-3’]; 2) for in vitro transcription assay; 3) for protein-protein interaction assay; and 4) for cell growth assay.

Quality Control:

Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.

Unit Definition:

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.

Concentration:

variable in different lots

References:

1. Pellegata, NS. et al., (1995) Oncogene 11, 337-349
2. El-Deiry, WS. et al., (1992) Nature Genet 1, 45-49
3. Hollstein, M. et al., (1991) Science 253, 49-53
4. Hupp, TR. et al., (1992) Cell 71, 875-886
5. Ishioka, C. et al., (1995) Oncogene 10, 1485-1492
6. Waterman, MJ. et al., (1996) Cancer Res 56, 158-163