p53 (wild type) Tumor Suppressor Protein and Transcription Factor, 5,000 units

Cat. No.   Description Price TP200114 P2001-01 p53 (wild type) Tumor Suppressor Protein and Transcription Factor, 5,000 units $ 275 The associated gene in an expression vector NM_000546 is also available.

Description:

p53 was identified as a tumor suppressor by showing to block the transformation and to inhibit tumor cell growth. In addition, this protein is a transcription factor capable of regulating the expression of a subset of downstream genes (1-5). Different mutations in p53 gene have been characterized in a variety of human cancers (6, 7). Loss or mutation of p53 function is highly correlated with tumorigenesis.

Source:

The wild type p53 (393 amino acids was expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography.

Applications:

Recombinant p53 can be used 1) for gel mobility shift assay or for a DNase I footprinting in the presence of double stranded DNA containing a consensus p53-binding sequence [5’-PuPuPuC(A/T)(T/A)GPyPyPy-3’]; 2) for in vitro transcription assay; 3) for protein-protein interaction assay; and 4) for cell growth assay.

Quality Control:

Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.

Unit Definition:

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.

Concentration:

variable in different lots

References:

1. Finlay, C. et al., (1989) Cell 57, 1083-1093
2. Michalovitz, D. et al., (1990) Cell 62, 671-680
3. Baker, S. et al., (1990) Science 249, 912-915
4. Fields, S. et.al., (1990) Science 249, 1046-1049
5. Raycroft, L. et al., (1990) Science 249, 1049-1051
6. Hollstein, M. et al., (1991) Science 253, 49-53
7. Bennett, W. et al., (1992) Chest 101, 19S-20S