EBI3 (NM_005755) Human Recombinant Protein

CAT#: TP723851

Purified recombinant protein of Human Epstein-Barr virus induced 3 (EBI3)

Product Images

Recombinant Human IL-27 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 1.5 - 9 ng/mL

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human IL-27, consisting two subunits, EBI3, the region of Arg21-Lys229, from gene Accession# NM_005755 , and p28, the region of Phe29-Pro243, from gene Accession# NM_145659, linked via a GS bridge
• Tag: C-His
• Predicted MW: 50 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 0.22 µm filtered protein solution is in 20 mM HEPES, 300 mM NaCl, 2 mM CHAPS, pH 7.2
• Bioactivity: The ED50 is 60 - 90 ng/ml, corresponding to a specific activity of 1.1 - 1.7 x 10^4 units/mg, determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
  Co-immunoprecipitation (PMID: 27019190)
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_005746
• Locus ID: 10148
• UniProt ID: Q14213
• Cytogenetics: 19p13.3
• Refseq Size: 1149
• Refseq ORF: 687
• Synonyms: IL-27B; IL27B; IL35B
• Summary: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]
• Protein Families: Druggable Genome, Secreted Protein