| Species: | Human |
Expression Host: | Sf9 cells |
| Expression cDNA Clone and Sequence |
A DNA sequence from TrueORF clone, RC400096, encoding human full-length Mutant IDH1(R132H) |
| Tag: | C-terminal DDK |
Predicted MW: | 47 kDa |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
| Concentration: | >50 ug/mL as determined by microplate BCA method |
| Buffer and Storage: | 50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. |
| BioActivity: |
Enzymatic activities were determined by monitoring NADPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and NADP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of NADP to NADPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are inactive. |
| Protein Families: |
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| Protein Pathways: |
Citrate cycle (TCA cycle)Glutathione metabolismMetabolic pathways |
