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Home Recombinant Human Proteins All IDH1 Proteins

IDH1 (NM_005896) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP710043 Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells., 20 ug (Larger pack size?) $399 In Stock
TA800426 IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, clone OTI24A2 (formerly 24A2) $379 In Stock
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OriGene Data
Species:Human Expression Host:Sf9 cells
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC400096, encoding human full-length Mutant IDH1(R132H)
Tag:C-terminal DDK Predicted MW:47 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
BioActivity: Enzymatic activities were determined by monitoring NADPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and NADP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of NADP to NADPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are inactive.
Protein Families:
Protein Pathways: Citrate cycle (TCA cycle)Glutathione metabolismMetabolic pathways
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_005887 RefSeq Size: 2339 RefSeq ORF: 1245
LocusID: 3417 Cytogenetic: 2q34  
Synonyms : HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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